APC anti-human CD276 (B7-H3) Antibody

Pricing & Availability
Clone
MIH42 (See other available formats)
Regulatory Status
RUO
Other Names
B7RP2
Isotype
Mouse IgG1, κ
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Product Citations
publications
MIH42_APC_CD276_Antibody_FC_101414
Human B7-H3 transfected P815 cells were stained with CD276 (clone MIH42) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • MIH42_APC_CD276_Antibody_FC_101414
    Human B7-H3 transfected P815 cells were stained with CD276 (clone MIH42) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
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351005 25 tests £80
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351006 100 tests £200
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Description

B7-H3, assigned as CD276, is a type I transmembrane protein and shares 20-27% amino acid identity with other B7 family members. Human B7-H3 has a single extracellular variable-type immunoglobulin (IgV)-IgC domain, a signature intracellular domain, and an additional isoform, known as 4Ig-B7-H3, containing nearly exact tandem duplication of the IgV-IgC domain and most likely caused by exon duplication. B7-H3 mRNA is broadly expressed in normal tissues whereas its protein expression is relatively rare. The expression of B7-H3 is induced on T cells, natural killer (NK) cells, and antigen-presenting cells (APCs), including dendritic cells (DCs) and macrophages. It can be upregulated during the maturation from monocytes to DCs, or during the interaction between DCs and regulatory T cells. B7-H3 has been shown to be a co-stimulatory molecule that inhibits T-cell responses. B7-H3 has also been identified to bind TLT-2 involved in the intracellular signaling pathway.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human B7-H3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
RRID
AB_2564403 (BioLegend Cat. No. 351005)
AB_2564404 (BioLegend Cat. No. 351006)

Antigen Details

Structure
Type I transmembrane protein with extracellular variable-type immunoglobulin domain and intracellular domain
Distribution

Dendritic cells, macrophages, natural killer cells, induced T cells

Function
Involved in intracellular signaling pathway
Interaction
Co-stimulator molecule inhibits T-cell response. Binds Trem like transcript 2 protein (TLT-2) to involve in intracellular signaling.
Ligand/Receptor
Trem like transcript 2 protein (TLT-2)
Cell Type
Dendritic cells, Macrophages, NK cells, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
Molecular Family
CD Molecules
Gene ID
80381 View all products for this Gene ID
UniProt
View information about CD276 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/20/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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