- DJR1 (See other available formats)
- Regulatory Status
- HCDM listed
- Other Names
- TRAIL-R1, Apo-2, CD261, TNFRSF10A
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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DR4 is 56 kD member 10A of the TNFR superfamily (TNFRSF10A), also known as TRAIL-R1, Apo-2, and CD261. It is expressed at low levels by activated T cells and some tumors. After TRAIL engagement, DR4 (TRAIL-R1), through activation of NF-κB, induces apoptosis in the TRAIL ligated cell.Product Details
- Human, African Green, Cynomolgus
- Antibody Type
- Host Species
- Extracellular domain of DR4-human IgG1 Fc fusion protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: The DJR1 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR4/TRAIL-R1 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307206) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
(PubMed link indicates BioLegend citation)
- Uno K, et al. 2003. Blood 101:3658.
- Sato K, et al. 2005. J. Immunol. 174:4025.
- Lundqvist A, et al. 2006. Cancer Res. 66:7317. PubMed
- Chen KF, et al. 2011. J Pharmacol Exp Ther. 337:155. PubMed
- Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed
- Chandrasekaran S, et al. 2014. PLoS One. 9:111487. PubMed
- Product Citations
AB_2256112 (BioLegend Cat. No. 307207)
AB_2204667 (BioLegend Cat. No. 307208)
- TNFR superfamily, 56 kD
Activated T cells, tumor cells
- Induces apoptosis
- Cell Type
- T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Macfarlane M. 2003. Toxicol. Lett. 139:89.
2. Baetu T, et al. 2002. Cytokine Growth Factor Rev. 13:199.
3. Degli-Esposti M. 1999. J. Leukoc. Biol. 65:535.
- Gene ID
- 8797 View all products for this Gene ID
- View information about CD261 on UniProt.org
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