APC anti-human Arginase I Antibody

Pricing & Availability
Clone
14D2C43 (See other available formats)
Other Names
ARG1, Liver Arginase, Type 1 Arginase, Arginase-1, Liver-Type Arginase
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
14D2C43_APC_Argniase-1_Antibody_081519.png
Human peripheral blood was surface stained with CD15 Pacific Blue™ and CD16 FITC, fixed, permeabilized, and then intracellularly stained with Arginase I (clone 14D2C43) APC (filled histogram) or mouse IgG2b, κ APC isotype control (open histogram). Histogram was gated on the neutrophil (CD15+CD16+) population.
  • 14D2C43_APC_Argniase-1_Antibody_081519.png
    Human peripheral blood was surface stained with CD15 Pacific Blue™ and CD16 FITC, fixed, permeabilized, and then intracellularly stained with Arginase I (clone 14D2C43) APC (filled histogram) or mouse IgG2b, κ APC isotype control (open histogram). Histogram was gated on the neutrophil (CD15+CD16+) population.
See APC spectral data
Cat # Size Price Quantity Avail. Save
369705 25 tests £81
Check Availability


Need larger quantities of this item?
Request Bulk Quote
369706 100 tests £195
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Arginase I, also known as ARG1, is a 34.7kD protein expressed by neutrophils and myeloid-derived suppressor cells (MDSCs). There are two isoforms that are differentiated based on their tissue distribution and subcellular localization. Arginase I converts L-arginine into L-ornithine and urea; it is the final enzyme in the urea cycle. While mostly found in the liver, it can also be expressed in cells lacking a comprehensive urea cycle. Also, it contributes to vasodilation and vascular function. Arginase I is also reported to be involved in MDSC mediated suppression of T cell proliferation.  Arginase I has been implicated in hyperargininemia (decreased function of arginase I) and Q fever.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant protein expressed in E.coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2814328 (BioLegend Cat. No. 369705)
AB_2814329 (BioLegend Cat. No. 369706)

Antigen Details

Structure
Belongs to the ureohydrolase family and has a molecular mass of 34.7 kD.
Distribution

Neutrophils and myeloid-derived suppressor cells (MDSC).

Function
Converts L-arginine into L-ornithine and urea.
Interaction
Arginine.
Cell Type
Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References

1. Munder M, et al. 2005. Blood 105:2549.
2. Luckner-Minden C, et al. 2010. JLB. 87:1125.
3. Holowatz L, et al. 2006. Journal of Physiology 574:573.
4. Sin YY, et al. 2013. PLoS One 8:11.
5. Benoit M, et al. 2008. Eur. J. Immunol. 4:1065.

Gene ID
383 View all products for this Gene ID
UniProt
View information about Arginase I on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/15/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account