Anti-Nestin Antibody (Previously Covance catalog# PRB-570C)

Pricing & Availability
Clone
Poly18419 (See other available formats)
Other Names
NES
Previously
Covance Catalog# PRB-570C
Isotype
Rabbit Polyclonal IgG
Ave. Rating
Submit a Review
Product Citations
publications
Poly18419_Nestin_Serum_IF_030315.jpg
Rabbit anti-human nestin staining of a human glioblastoma cell line, U251. Nestin is stained in green, blue is bisbenzimide. Photo courtesy of J. Hou, NIH.
  • Poly18419_Nestin_Serum_IF_030315.jpg
    Rabbit anti-human nestin staining of a human glioblastoma cell line, U251. Nestin is stained in green, blue is bisbenzimide. Photo courtesy of J. Hou, NIH.
Cat # Size Price Quantity Avail. Save
841901 100 µl £218
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Nestin, a class IV intermediate filament protein is expressed during early stages of development in the CNS and in myogenic tissues. Upon differentiation, nestin becomes down regulated and is replaced by other tissue specific intermediate filaments. Antibodies against nestin can be used as a predominant marker to describe stem and neural progenitor cells in the mammalian CNS.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
The polyclonal nestin antibody was raised using a GST fusion protein.
Preparation
Serum
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

ICC, WB, IHC, IF

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:5,000*
IHC: 1:200
IF: 1:200

Application Notes

This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC) and immunohistochemistry (IHC) applications.

*Predicted MW = 220-240 kD

Using this antiserum, nestin expression was detected in progenitor cells and in a small subset of neurons. In addition, nestin was also detected in cells that were in transition from a progenitor stage to glial differentiation.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Messam C, Hou J, Major E. Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody. Exp. Neurol. 161:585-596, 2000.
  2. Ueno M, et al. 2006. PNAS. 103:9554.
  3. Nagase T, et al. 2009. Dev Dyn. 238:1118.
  4. Watanabe K, et al. 2007. Nat Biotechnol. 25:681.
  5. Sosunov A, Guilfoyle E, Wu X, McKhann G, Goldman J. Phenotypic Conversions of Protoplasmic" to "Reactive" Astrocytes in Alexander Disease. J. Neurosci.; 33: 7439 - 7450, Apr 2013. [IF] PubMed
Product Citations
  1. Sosunov A, et al. 2013. J Neurosci. 33:7439-7450. PubMed
  2. Momcilovic O, et al. 2016. PLoS One. 11: 0154890. PubMed
Publication Library
RRID
AB_2565468 (BioLegend Cat. No. 841901)

Antigen Details

Cell Type
Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Intermediate Filaments
Gene ID
10763 View all products for this Gene ID
UniProt
View information about Nestin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/14/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account