- LaM-4 (See other available formats)
- Regulatory Status
- Other Names
- Llama VH Ig
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- Product Citations
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mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. It is the most widely used photostable protein in the class of red-shifted fluorescent proteins. mCherry derives from proteins initially isolated from Cnidarians.
Nanobodies are heavy chain only, single-domain antibodies, that lack the presence of light chains. These are typically derived from members of the Camelidae family that include llamas and camels. Their variable region (VHH) is the smallest antigen-binding fragment found in a natural antibody, and nanobodies are the smallest (≈15 kD) naturally occuring immunoglobins. Further, nanobodies are stable, and can bind antigens with high affinity. Experimentally, nanobodies have been applied in WB, IF, and IP. In IP/WB applications, nanobodies can avoid IgG heavy/light chain issues that occur when using regular antibodies.
- Verified Reactivity
- Discosoma mCherry
- Antibody Type
- Recombinant mCherry.
- 50% anti-mCherry nanobody (clone LaM-4) conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
- The antibody was purified by affinity chromatography.
- Storage & Handling
- Upon receipt, store between 2°C and 8°C. The unopened product is stable for one year upon arrival.
IP - Quality tested
- Recommended Usage
For immunoprecipitation, the suggested use of this reagent is 5 - 20 µl affinity gel for 100 µg lysate harvested from mCherry overexpressed cells.
- mCherry is 236 amino acids with a predicted molecular weight of approximately 27 kD.
- Fluorescent protein.
- Biology Area
- Cell Biology
- Antigen References
1. Shaner NC, et al. 2008. Nat. Methods. 6:545-51.
2. Shaner NC, et al. 2005. Nat. Methods 12:905-9.
3. Cortez-Retamozo V, et al. 2004. Cancer Res. 64:2853.
4. Muyldermans S. 2013. Annu. Rev. Biochem. 82:775.
5. Fridy PC, et al. 2014. Nat. Methods 11:1253.
- Gene ID
- View information about mCherry on UniProt.org
|Anti-mCherry Nanobody Affinity Gel||LaM-4||IP|
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