Anti-Giantin Antibody (Previously Covance catalog# PRB-114C)

Pricing & Availability
Clone
Poly19243 (See other available formats)
Other Names
GCP, GCP372, GOLIM1, Golgin Subfamily B Member 1, Macrogolgin, Golgi Integral Membrane Protein 1, 372 kD Golgi Complex-Associated Protein, Golgin B1, Golgi Autoantigen, Golgin Subfamily B, Macrogolgin (With Transmembrane Signal)
Previously
Covance Catalog# PRB-114C
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Purified_Giantin_Antibody_IF_030415
HeLa cells stained with polyclonal Giantin (green) used at 1:500 and monoclonal TU27 (red) used at 1:2000.
  • Purified_Giantin_Antibody_IF_030415
    HeLa cells stained with polyclonal Giantin (green) used at 1:500 and monoclonal TU27 (red) used at 1:2000.
Cat # Size Price Quantity Avail. Save
924301 25 µl £50
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924302 200 µl £202
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Description

Giantin is a membrane-inserted component of the cis and medial Golgi with a large rod-like cytoplasmic domain. This antibody serves as an excellent marker for the Golgi complex in mammalian cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Monkey, Mouse, Rat, Rodent
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This polyclonal antibody was raised against the N-terminus, residues 1-469, of human giantin
Preparation
Serum
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

IF, ICC

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry.

The optimal working dilution should be determined for each specific assay condition.
IF: 1:1,000

Application Notes

This antibody is effective in immunoblotting (WB) and immunofluorescence (IF).

*Predicted MW = 367 kD. Western transfer should be conducted in the presence of 0.1% SDS. After transfer, rinse blot with 50% MeOH to remove any residual SDS.

Giantin is a membrane-inserted component of the cis and medial Golgi with a large rod-like cytoplasmic domain. This antibody serves as an excellent marker for the Golgi complex in mammalian cells.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Day PM, et al. 2013. J. Virol. 87: 3862. (IF) PubMed
  2. Linstedt AD, Hauri HP. 1993. Mol. Biol. Cell. 4:679.
  3. Asante D, et al. 2013. J. Cell. Sci. 126:5189. (WB) PubMed
  4. Cohen RM, et al. 2013. J. Neurosci. 15:6245. (WB) PubMed
Product Citations
  1. Schwartz V, et al. 2015. Sci Rep. 5: 18242. PubMed
  2. Day P, et al. 2013. J Virol. 87:3862-3870. PubMed
  3. Asante D, et al. 2013. J Cell Sci. 126:5189-5197. PubMed
  4. Cohen R, et al. 2013. J Neurosci. 33:6245-6256. PubMed
  5. González C, et al. 2016. Proc Natl Acad Sci U S A. 113: 1823 - 1828. PubMed
  6. Engelke M, et al. 2016. Nat Commun. 7: 11159. PubMed
  7. Vilmont V, et al. 2016. Sci Rep. 6:27804. PubMed
  8. Tian X, et al. 2016. J Biol Chem. 291: 14510 - 14525. PubMed
  9. Gershlick D, et al. 2016. Mol Biol Cell. 27: 2867 - 2878. PubMed
  10. Emnett C, et al. 2016. Sci Rep. 6:38808. PubMed
  11. Fernandez-Fernandez M, et al. 2017. J Cell Sci. 130(1):83-89. PubMed
  12. Wesolowski J, et al. 2017. mBio. 10.1128/mBio.02280-16. PubMed
  13. Hoyer MJ 2018. Cell. 175:254. PubMed
Publication Library
RRID
AB_2565451 (BioLegend Cat. No. 924302)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Golgi Markers
Gene ID
2804 View all products for this Gene ID
UniProt
View information about Giantin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04/15/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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