Anti-β-Amyloid, 1-16 Antibody (Previously Covance catalog# SIG-39300)

Pricing & Availability
Clone
6E10 (See other available formats)
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9300-02
Signet Catalog# 9300-05
Signet Catalog# 9300-10
Covance Catalog# SIG-39300
Isotype
Mouse IgG1
Ave. Rating
2 reviews
Product Citations
publications
A_6E10_ASCITES_betaAmyloid_1-16_WB_050818
Western blot of anti-β-amyloid, 1-16 antibody (clone 6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of the human Aβ1-40 peptide; Lane 3: 50 ng of the Aβ1-42 peptide; Lane 4: 50 ng of the recombinant human APP751 protein; Lane 5: 20 µg of the human brain lysate; Lane 6: 50 ng of the rodent Aβ1-42 peptide. The blot was incubated with 1:10,000 dilution of the primary antibody overnight at 4°C, followed by incubation with the HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A_6E10_ASCITES_betaAmyloid_1-16_WB_050818
    Western blot of anti-β-amyloid, 1-16 antibody (clone 6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of the human Aβ1-40 peptide; Lane 3: 50 ng of the Aβ1-42 peptide; Lane 4: 50 ng of the recombinant human APP751 protein; Lane 5: 20 µg of the human brain lysate; Lane 6: 50 ng of the rodent Aβ1-42 peptide. The blot was incubated with 1:10,000 dilution of the primary antibody overnight at 4°C, followed by incubation with the HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • B_6E10_ASCITES_betaAmyloid_1-16_DirectELISA_050818
    Direct ELISA of anti-β-amyloid, 1-16 (clone 6E10) antibody binding to the plate-immobilized human Aβ1-40, human Aβ1-42, rodent Aβ1-42 peptides, and recombinant human APP751 protein. ELISA was performed by coating the wells with 100 ng of peptide or recombinant protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with HRP labeled goat anti-mouse IgG secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
  • C_6E10_ASCITES_betaAmyloid_1-16_IHCP_050818
    IHC staining of anti-β-Amyloid, 1-16 antibody (clone 6E10) on formalin-fixed paraffin-embedded normal human (left panel) and Alzheimer’s disease (right panel)brain tissues. Following antigen retrieval using 70% formic acid for 20 minutes, the tissues were incubated with 1:5,000 dilution of the primary antibody for 1 hour at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
803014 50 µl £171
Check Availability


Need larger quantities of this item?
Request Bulk Quote
803015 200 µl £604
Check Availability


Need larger quantities of this item?
Request Bulk Quote
803016 500 µl £1,229
Check Availability


Need larger quantities of this item?
Request Bulk Quote
803017 1 ml £2,060
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling
Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.
Application

WB - Quality tested
Direct ELISA, IHC-P - Validated
IHC-F, EM - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution of this reagent is 1:1,000 to 1:20,000. For Direct ELISA, the suggested dilution of this reagent is 1:10,000 to 1:100,000. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested dilution of this reagent is 1:1,000 to 1:20,000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to amino acid residue 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS).

This antibody clone has been reported for use in immunohistochemistry of free-floating sections2,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Thakker DR, et al. 2009. Proc. Natl. Acad. Sci. USA. 106(11):4501-6. (IHC) PubMed
  2. Oddo S, et al. 2005. Proc. Natl. Acad. Sci. USA. 102(8):3046-51. (IHC-other) PubMed
  3. Herzig M, et al. 2004. Nat. Neuro. 7(9):954-959. (WB) PubMed
  4. Zheng Y, et al. 2012. PLoS One 6:39035. (IHC-F) PubMed
  5. Abramowksi D, et al. J Neurosci. 32:1273. (WB) PubMed
  6. Forny-Germano L, et al. 2014. J. Neurosci. 34:13629. (WB, IHC) PubMed
  7. Gowert NS, et al. 2014. PLoS One 2:e90523. (ICC, EM) PubMed
  8. Sandoval-Hernández A, et al. 2015. PLoS One. 10: 0145467. (IHC-F)
  9. Kumar R, et al. 2016. Brain. 139:174-92 (WB)
  10. Miyamoto T, et al. 2016. J. Biol. Chem. 291:1719-34. (WB)
  11. Saito S, et al. 2017. Acta Neuropathol. Commun. 5:26-9. (IHC-P) PubMed
  12. Omata Y, et al. 2016. Aging (Albany NY) 8(3):427. (IHC-P) PubMed
  13. Peng W, et al. 2016. Neurobiol. Dis. 93:215. (IHC-other) PubMed
  14. Mandler M, et al. 2015. PLoS One. e0115237. (WB, IHC, ELISA) PubMed
Product Citations
  1. Klohs J, et al. 2016. J Cereb Blood Flow Metab. 36: 1614 - 1624. PubMed
  2. Zheng Y, et al. 2012. PLoS One. 7:e39035. PubMed
  3. Forny-Germano L, et al. 2014. J Neurosci. 34:13629-13643. PubMed
  4. Gowert N, et al. 2014. PLoS One. 9:e90523. PubMed
  5. Abramowski D, et al. 2012. J Neurosci. 32:1273-1283. PubMed
  6. Chan E, et al. 2016. Sci Rep. 6: 26119. PubMed
  7. Heiss J, et al. 2016. Cereb Cortex. 10.1093/cercor/bhw188. PubMed
  8. Li T, et al. 2016. Nat Commun. 7:12082. PubMed
  9. Fujita K, et al. 2016. Sci Rep. 6:31895. PubMed
  10. Beraldo F, et al. 2016. J Biol Chem. 291: 21945 - 21955. PubMed
  11. Zhang H, et al. 2016. J Neurosci. 36: 11837 - 11850. PubMed
  12. Scholtzova H, et al. 2017. J Neurosci. 37(4):936-959. PubMed
  13. Jang J, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.08.127. PubMed
  14. Casali BT, et al. 2018. J Neuroinflammation. 15:43. PubMed
  15. Fu W, et al. 2018. Exp Ther Med. 128:2252. PubMed
  16. Lee CYD 2018. Neuron. 97:1032. PubMed
  17. Boeddrich A 2018. Cell chemical biology. 26(1):109. PubMed
  18. Williamson RL, et al. 2017. J Biol Chem. 292:19873. PubMed
  19. Cheng WH, et al. 2019. Alzheimers Res Ther. 11:6. PubMed
  20. Li N, et al. 2019. Neural Regen Res. 14:1037. PubMed
Publication Library
RRID
AB_2728527 (BioLegend Cat. No. 803014)
AB_2565328 (BioLegend Cat. No. 803015)
AB_2565329 (BioLegend Cat. No. 803016)
AB_2565327 (BioLegend Cat. No. 803017)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1
  3. Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
  4. Puig KL, et al. 2012.  Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ, et al. 2016. EMBO Mol. Med. 8(6):595.
  6. Walsh DM, et al.  2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid, 1-16 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 05/08/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account