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Alexa Fluor® 700 anti-mouse FOXP3 Antibody

Pricing & Availability
Clone
MF-14 (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
MF-14_A700_FOXP3_Antibody_090718
C57BL/6 splenocytes were surface stained with CD4 PE and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone MF-14) Alexa Fluor® 700 (left) or Rat IgG2b, κ Alexa Fluor® 700 isotype control (right).
  • MF-14_A700_FOXP3_Antibody_090718
    C57BL/6 splenocytes were surface stained with CD4 PE and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with FOXP3 (clone MF-14) Alexa Fluor® 700 (left) or Rat IgG2b, κ Alexa Fluor® 700 isotype control (right).
Compare all formats See Alexa Fluor® 700 spectral data
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126421 25 µg £117
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126422 100 µg £257
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Description

FOXP3 is a 47 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Ono M, et al. 2007. Nature 446:685.
  2. Hori S, et al. 2003. Science 299:1057.
  3. Fontenot JD, et al. 2003 Nature Immunol 4:330.
  4. Fallarino F, et al. 2009. J. Immunol. 183:6033. PubMed
  5. Barber A, et al. 2009 J. Immunol. 183:6939. PubMed
  6. Nakashima H, et al. 2010. J. Immunol. 184:4637. PubMed
RRID
AB_2750492 (BioLegend Cat. No. 126421)
AB_2750493 (BioLegend Cat. No. 126422)

Antigen Details

Structure
50-55 kd protein. Forkhead/winged-helix transcription factor family, contains zinc finger and forkhead domains.
Distribution

Nuclear; expressed in Treg cells.

Function
Master regulatory gene in Treg cell development, crucial for immune homeostasis.
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057.
3. Fontenot JD, et al. 2003 Nature Immunol 4:330.

Regulation
Present at high level in T reg cells. Induced by T cell activation.
Gene ID
20371 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org

Related FAQs

Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?

It is not recommended. It is best to use PBMCs for this testing.

Can FOXP3 be costained with cytokines?

The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.

Go To Top Version: 1    Revision Date: 09/10/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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