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Alexa Fluor® 700 anti-mouse CD63 Antibody

Pricing & Availability
Clone
NVG-2 (See other available formats)
Regulatory Status
RUO
Other Names
LIMP, LAMP-3, gp55, melanoma-associated antigen, ME491
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
NVG-2_A700_CD63_Antibody_011320
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed, permeabilized and intracellularly stained with CD11b PE and CD63 (clone NVG-2) Alexa Fluor® 700 (left) or Rat IgG2a, κ Alexa Fluor® 700 isotype control (right).
  • NVG-2_A700_CD63_Antibody_011320
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed, permeabilized and intracellularly stained with CD11b PE and CD63 (clone NVG-2) Alexa Fluor® 700 (left) or Rat IgG2a, κ Alexa Fluor® 700 isotype control (right).
  • NVG-2_A700_CD63_Antibody_2_040620
    bEnd.3, mouse endothelial cells were stained with CD63 (clone NVG-2) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
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143923 25 µg £109
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143924 100 µg £217
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Description

CD63, also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491), is a member of the tetraspanin superfamily (TM4SF) that constitutes a main component of the lysosomal membrane. It is expressed on activated platelets, monocyte/macrophages, endothelium, fibroblasts, osteoblasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6. In mice, there are two CD63 gene loci, of which only one is functional. CD63 deficient mice are viable, and there is no alteration in the population of immune cells.  A recent report shows that CD63-deficient mice exhibit a significant reduction in both leukocyte rolling and recruitment in a peritonitis model. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Intestinal lamina propria light-density cells (enriched with eosinophils)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1 and immunofluorescence1.

Application References

(PubMed link indicates BioLegend citation)
  1. Verjan Garcia N, et al. 2011. J. Immunol. 187:2268. (WB, IF)
RRID
AB_2832515 (BioLegend Cat. No. 143923)
AB_2860699 (BioLegend Cat. No. 143924)

Antigen Details

Structure
Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Distribution

Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells

Function
Platelet activation
Interaction
CD9, CD81, VLA-3, and VLA-6
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Azorsa DO, et al. 1991. Blood 78:280.
2. Kishimoto T, et al. 1997. Leukocyte Typing V1. Oxford University Press New York.
3. Hildreth JE, et al. 1991. Blood 77:121.

Gene ID
12512 View all products for this Gene ID
UniProt
View information about CD63 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/06/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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