- SMI 81 (See other available formats)
- Other Names
- Synaptosomal-associated protein 25, SUP, Synaptosomal-associated 25 kD Protein
- Mouse IgG1, κ
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- Product Citations
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Synaptosomal-associated protein 25 (SNAP-25) is a component of the trans-SNARE (t-SNARE) complex. SNAP-25 has been reported to account for the specificity of membrane fusion, and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP-25 has been shown to inhibits both ATP-dependent and independent Ca2+-triggered release of glutamate from central nervous system (CNS) synaptosomal membranes. This indicates that SNAP-25 has a role not only in the formation of the synaptic vesicle-target v- and t-SNARE complex, but also in the final neurotransmitter release.Product Details
- Human, Mouse, Rat
- Antibody Type
- Host Species
- Full length, unaltered SNAP-25.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-P - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 2.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
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- Excitation Laser
Red Laser (633 nm)
(PubMed link indicates BioLegend citation)
- Postupna NO, et al. 2014. Lab Invest. 94:1161. (FC)
- Hirano AA, et al. 2011. J. Comp. Neurol. 519:972. (WB, IHC, EM) PubMed
- Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
- Rozas JL, et al. 2012. Neuron. 74:151. (IF) PubMed
- Brinkmalm A, et al. 2014. Mol. Neurodegener. 9:53. (IP) PubMed
- Gu Y, et al. 2016. Proc. Natl. Acad. Sci. U. S. A. E922-31. (WB) PubMed
- Kabachinski G, et al. 2016. Mol. Biol. Cell. 654-68.(IF) PubMed
- Kunii M, et al. 2016. J. Cell Biol. 215:121-138. (IP) PubMed
- SNAP-25 is a 206 amino acid protein with a molecular mass of 23 kD.
Tissue distribution: Mainly in the central nervous system, in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Cellular distribution: Cytosol, golgi apparatus, and cell membrane.
- SNAP-25 associates with proteins involved in vesicle docking and membrane fusion. It regulates plasma membrane recycling through its interaction with centromere protein F (CENPF).
- In adult tissue, SMI 81 visualizes synapse-rich areas of brain and retina. In developing brain and cell cultures, SMI 81 reacts with neuronal cell bodies and axons. It is useful as an early marker for developing neurons in cell culture.
- Cell Type
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
- Molecular Family
- Presynaptic proteins
- Antigen References
1. Snyder DA, et al. 2006. Cell Biochem. Biophys. 45(1):111-23.
2. Nagy G, et al. 2004. Neuron. 41(3):417–29.
- Gene ID
- 6616 View all products for this Gene ID
- View information about SNAP-25 on UniProt.org
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