Alexa Fluor® 647 anti-mouse TCR Vβ10 (a haplotype) Antibody

Pricing & Availability
Clone
8.48 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vb10
Isotype
Mouse IgG1, κ
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Product Citations
publications
8dot48_A647_090808.jpg
SJL splenocytes stained with CD3 (145-2C11) PE and 8.48 Alexa Fluor® 647
  • 8dot48_A647_090808.jpg
    SJL splenocytes stained with CD3 (145-2C11) PE and 8.48 Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
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128903 25 µg £97
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Description

This antibody reacts with Vb10 TCR of mice having the a haplotype (SJL, C57BR, SWR, C57L), but not with TCRb (e.g., AKR, BALB/c C3H, C57BL, DBA/1, DBA/2) or TCRc haplotypes (e.g., RIII).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
1B6 Rag2 knockout transgenic T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_1236445 (BioLegend Cat. No. 128903)

Antigen Details

Structure
TCR consists of alpha and beta chain. This antibody recognizes Vb 10 of TCR a haplotype (e.g. SJL, C57L, SWR and C57BR).
Distribution

Positive on a subset of alpha/beta T cells from mice of TCR a haplotype.

Ligand/Receptor
MHC/peptide
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Necker A, et al. 1991. Eur. J. Immunol. 21:3035

Gene ID
100124660 View all products for this Gene ID
UniProt
View information about TCR Vbeta10 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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