Alexa Fluor® 647 anti-mouse Ly49H Antibody

Pricing & Availability
3D10 (See other available formats)
Regulatory Status
Other Names
Klra8, Cmv1
Mouse IgG1, κ
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Product Citations
3D10_AF647_Ly49H _Antibody_FC_1_010813
C57BL/6 mouse splenocytes were stained with CD49b/DX5 Pacific Blue™ and Ly49H (clone 3D10) Alexa Fluor® 647 (top) or mouse IgG1, ® isotype control Alexa Fluor® 647 (bottom).
  • 3D10_AF647_Ly49H _Antibody_FC_1_010813
    C57BL/6 mouse splenocytes were stained with CD49b/DX5 Pacific Blue™ and Ly49H (clone 3D10) Alexa Fluor® 647 (top) or mouse IgG1, ® isotype control Alexa Fluor® 647 (bottom).
  • 3D10_AF647_Ly49H _Antibody_FC_2_010813
Compare all formats See Alexa Fluor® 647 spectral data
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144709 25 µg £58.00
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144710 100 µg £144.00
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Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein.  It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not Balb/c or DBA/2 mice).  Expression of Ly49H has been associated with resistance to MCMV infection in C57BL/6 mice.  Modulation of Ly49H expression by its antibody made MCMV-resistant C57BL/6 mice susceptible to infection.  Unlike Ly49A, Ly49H lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) but has ITAM (immunoreceptor tyrosine-based activation motif), which is associated with DAP-12 (KARAP, killer cell activating motif).

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
C1498-Ly49A/H transfectant
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipation1, and in vitro2 and in vivo3 blocking activity.  The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 144717 & 144718).

Application References

(PubMed link indicates BioLegend citation)
  1. Guseva NV, et al. 2010. Eur. J. Immunol. 40:2618. (IP)
  2. Orr MT, et al. 2009. J. Exp. Med. 206:807. (Block)
  3. Rodriguez M, et al. 2004. J. Immunol. 173:6312. (Block)
Product Citations
  1. Ranganathan P, et al. 2013. J Am Soc Nephrol. 25:239. PubMed
AB_2561745 (BioLegend Cat. No. 144709)
AB_2561746 (BioLegend Cat. No. 144710)

Antigen Details

28.3 kD

NK cells but not NKT cells

Interacts with m157, a cytomegalovirus-encoded protein
Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Gosselin P, et al. 1999. J. Leuko. Bio. 66:165.
2. Lee SH, et al. 2001. Nat. Genet. 28:42.
3. Daniels KA, et al. 2001. J. Exp. Med. 194:29.

Gene ID
16639 View all products for this Gene ID
View information about Ly49H on

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Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse Ly49H

  • Purified anti-mouse Ly49H

  • PE anti-mouse Ly49H

  • Alexa Fluor® 647 anti-mouse Ly49H

  • PE/Cyanine7 anti-mouse Ly49H

  • APC anti-mouse Ly49H

  • TotalSeq™-A0839 anti-mouse Ly49H

  • Ultra-LEAF™ Purified anti-mouse Ly49H

  • TotalSeq™-C0839 anti-mouse Ly49H

  • TotalSeq™-B0839 anti-mouse Ly49H

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