Alexa Fluor® 647 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
1_BM8_A647_F4slash80_Antibody_1_FC_060616
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 647.
  • 1_BM8_A647_F4slash80_Antibody_1_FC_060616
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 647.
  • 2_BM8_A647_F4slash80_Antibody_2_IHCF_120320
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of anti-mouse F4/80 (clone BM8) Alexa Fluor® 647 (red), anti-mouse/human CD45R/B220 (clone RA3-6B2) Brilliant Violet 421™ (blue), and anti-mouse/rat/human CD27 (clone LG.3A10) Alexa Fluor® 594 (green) overnight at 4°C. The image was captured with a 10X objective.
  • 3_BM8_A647_F4-80_Antibody_2_092121.png
    Paraformaldehyde-fixed (1%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse I-A/I-E Antibody (clone M5/114.15.2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD146 Antibody (clone ME-9F1) Alexa Fluor® 594 at 5 µg/mL (orange), and anti-mouse F4/80 Antibody (clone BM8) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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See Alexa Fluor® 647 spectral data
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123121 25 µg £86.00
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123122 100 µg £188.00
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg/mL. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
Product Citations
  1. Wang S, et al. 2017. Cell Death Dis. 10.1038/cddis.2017.104. PubMed
  2. Gwela A, et al. 2017. J Crohns Colitis. 10.1093/ecco-jcc/jjx050. PubMed
  3. Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038/s41598-017-10135-1. PubMed
  4. Lin YW, et al. 2018. Sci Rep. 8:834. PubMed
  5. Bai WJ, et al. 2018. Front Immunol. 8:1919. PubMed
  6. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  7. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  8. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  9. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  10. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
  11. Qiao S, et al. 2019. J Neuroinflammation. 16:4. PubMed
  12. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  13. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  14. Garg BK, et al. 2019. PLoS One. 14:e0214942. PubMed
  15. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  16. Naito H, et al. 2019. Dev Cell. 48:151. PubMed
  17. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  18. Badenes M, et al. 2020. Mol Metab. 31:67. PubMed
  19. Galvani E, et al. 2020. Nat Commun. 11:853. PubMed
  20. Huai W, et al. 2019. J Exp Med. 216:772. PubMed
  21. Hosaka K, et al. 2020. Nat Commun. 3.030555556. PubMed
  22. Petrus P, et al. 2020. Cell Metabolism. 31(2):375-390.e11.. PubMed
  23. Ruiz–Manzano RA, et al. 2020. Frontiers in Oncology. 0.927777778. PubMed
  24. Dobrian A, et al. 2011. Am J Physiol Endocrinol Metab. 300:E410. PubMed
  25. Tavares J, et al. 2013. J Exp Med. 210:905. PubMed
  26. Jaworska K, et al. 2015. J Immunol. 194:325. PubMed
  27. Wang J, et al. 2015. Cancer Res. 75:306. PubMed
  28. Yang X, et al. 2015. Proc Natl Acad Sci U S A. 112:2900. PubMed
  29. Belliere J, et al. 2015. J Am Soc Nephrol. 26:1363. PubMed
  30. Shinohara M 2015. J Immunol. 194:5595. PubMed
  31. Hu K, et al. 2015. PLoS One. 10: 0137123. PubMed
  32. Shankar J 2016. Innate Immunity. 22: 31 - 39. PubMed
  33. Nocera D, et al. 2016. J Immunol. 196: 2860 - 2869. PubMed
  34. Marneros A 2016. EMBO Mol Med. 8: 208 - 231. PubMed
  35. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  36. Yang Y, et al. 2016. Nat Commun. 7:11385. PubMed
  37. Jiang H, et al. 2016. J Immunol. 197: 2880 - 2890. PubMed
  38. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  39. Chen J, et al. 2017. Sci Rep. 10.1038/s41598-017-00654-2. PubMed
  40. Dean JM, et al. 2020. Cell Rep. 33:108228. PubMed
  41. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  42. Woods PS, et al. 2020. Am J Respir Cell Mol Biol. 62:243. PubMed
  43. Shan Z, et al. 2021. eLife. 10:00. PubMed
  44. Marneros AG, et al. 2020. Developmental Cell. 54(3):348-366.e5. PubMed
  45. McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed
  46. Wang Y, et al. 2020. Immunity. 53(6):1168-1181.e7. PubMed
  47. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  48. Barkaway A, et al. 2021. Immunity. . PubMed
RRID
AB_893492 (BioLegend Cat. No. 123121)
AB_893480 (BioLegend Cat. No. 123122)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 8    Revision Date: 09/21/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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