- In1/CD74 (See other available formats)
- Regulatory Status
- Other Names
- CLIP, DHLAG, HLADG
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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CD74, also known as CLIP, is a 33.5kD homotrimer and functions as a MHC class II chaperone for exogenous peptides and other MHC class II molecules. CD74 binds MIF and regulates innate and adaptive immunity through CD44 signaling. It is found primarily on B-cells, monocytes, macrophages and dendritic cells, but can also be found on activated T-cells, endothelial and epithelial cells.Product Details
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
This clone is also known as In1 and In-1. Additional reported applications (for the relevant formats) include: immunohistochemistry1, immunoprecipitation3, and western blotting4.
(PubMed link indicates BioLegend citation)
- Momburg F, et al. 1986. J. Immunol. 136:940. (IHC)
- Takahashi K, et al. 2009. Respir. Res. 10:33. (FC)
- Meza-Romero R, et al. 2014. J. Immunol. 192:4164. (IP)
- Heinrichs D, et al. 2011. Proc. Natl. Acad. Sci. USA 108:17444. (WB)
- Product Citations
AB_2632608 (BioLegend Cat. No. 151003)
AB_2632609 (BioLegend Cat. No. 151004)
- 33.5 kD and homotrimer.
B-cells, monocytes/macrophages, dendritic cells, activated T-cells, and activated endothelial/epithelial cells.
- MHC class II chaperone, MHC class II molecule sorting, and peptide loading.
- MHC class II molecules and peptides.
- Macrophage migration inhibitory factor (MIF).
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, T cells
- Biology Area
- Molecular Family
- CD Molecules, MHC Antigens
- Antigen References
1. Herrero L, et al. 2013. Arthritis Rheum. 65:2724.
2. Geneve L, et al. 2012. Int. Immunol. 24:645.
3. Sille FC, et al. 2011. Immunol Lett. 139:33.
4. Shih NY, et al. 1995. Mol. Immunol. 32:643.
5. Shi X, et al. 2006. Immunity 25:595.
6. Starlets D, et al. 2006. Blood 107:4807.
- Gene ID
- 16149 View all products for this Gene ID
- View information about CD74 on UniProt.org
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