Alexa Fluor® 647 anti-mouse CD38 Antibody

Pricing & Availability
Clone
90 (See other available formats)
Regulatory Status
RUO
Other Names
T10
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A-90_Alx647_011108
C57BL/6 mouse splenocytes stained with 90 Alexa Fluor® 647
  • A-90_Alx647_011108
    C57BL/6 mouse splenocytes stained with 90 Alexa Fluor® 647
  • B-90_A647_CD38_Antibody_IHCF_051419
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS+ rat/mouse/hamster serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of Alexa Fluor® 647 anti-mouse CD38 (red) (Clone 90), 5 µg/ml Alexa Fluor® 488 anti-mouse CD3ε (green) (clone 145-2C11), and 5 µg/ml of Alexa Fluor® 594 anti-mouse F4/80 Antibody (blue) (Clone BM8) overnight at 4°C. The image was captured by 10X objective.
See Alexa Fluor® 647 spectral data
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102716 100 µg £133.00
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Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow pre-B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed
  2. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  3. Tan AHY, et al. 2019. Front Immunol. 10:1351. PubMed
  4. Zhang Y, et al. 2018. J Exp Med. 215:1227. PubMed
  5. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
RRID
AB_2073334 (BioLegend Cat. No. 102716)

Antigen Details

Structure
ADP-ribosyl hydrolase, 42 kD
Distribution

B cells, NK cells, T cell subset, brain, muscle, kidney

Function
Regulation of cellular activation/proliferation, adhesion, metabolism of cADPR and NAADP
Ligand/Receptor
CD31, hyaluronic acid
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Shubinsky G, et al. 1997. Immunity 7:315.
3. Cesano A, et al. 1998. J. Immunol. 160:1106.
4. Cockayne DA, et al. 1998. Blood 92:1324.

Gene ID
12494 View all products for this Gene ID
UniProt
View information about CD38 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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