- Ki-67 (See other available formats)
- Regulatory Status
- Other Names
- Antigen Ki-67
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Antigen Ki-67 is a nuclear protein expressed as two isoforms with molecular weights of 395 and 345 kD. Both isoforms contain one forkhead-associated domain and 16 concatenated "Ki-67 repeats," each containing the epitope recognized by the mAb Ki-67. The antigen Ki-67 interacts with Hklp2, hNIFK, and chromobox protein homolog 1, 3, and 5. Ki-67 is required for cell proliferation and its expression is restricted to the phases G1, S, G2, and M of the cell cycle. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. Ki-67 has also been used to study myocyte proliferation after myocardial infarction as well as lymphocyte proliferation during infection, and has been used in neurons of patients with different neuropathologies.Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- Nuclei of the Hodgkin lymphoma cell line L428
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunocytochemistry, a concentration of 5.0 µg/ml is recommented. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.
Ki-67 Staining Protocol:
1. Prepare 70% ethanol and chill at -20°C.
2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.
3. Discard supernatant and loosen the cell pellet by vortexing.
4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.
5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.
6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.
7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.
8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.
(PubMed link indicates BioLegend citation)
- Gerdes J, et al. 1983. Int. J. Cancer 31:13. (IHC)
- Gerdes J, et al. 1984. J. Immunol. 133:1710. (ICFC)
- Schluter C, et al. 1993 J. Cell Biol. 123:513. (IHC, WB)
- Bading H, et al. 1989 Exp. Cell. Res. 185:50. (IF)
- Guha P, et al. 2013. PNAS. 110:5052. PubMed
- Product Citations
AB_10900810 (BioLegend Cat. No. 350509)
AB_10900821 (BioLegend Cat. No. 350510)
- Two isoforms with molecular weights of 395 and 345 kD, one forkhead-associated domain, 16 concatenated Ki-67 repeats, located in nucleus
Expressed in the phases G1, S, G2, and M of the cell cycle
- Required for cell proliferation
- Chromobox protein homolog 1, 3 and 5, Hklp2, and hNIFK
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
- Molecular Family
- Nuclear Markers
- Antigen References
1. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
2. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
3. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
4. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
5. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
- Gene ID
- 4288 View all products for this Gene ID
- View information about Ki-67 on UniProt.org
Related Pages & Pathways
Other FormatsView All Ki-67 Reagents Request Custom Conjugation
|Brilliant Violet 510™ anti-human Ki-67||Ki-67||ICFC,ICC|
|Purified anti-human Ki-67||Ki-67||ICFC,CyTOF®,ICC,WB,IHC-F|
|PE anti-human Ki-67||Ki-67||ICFC|
|Brilliant Violet 421™ anti-human Ki-67||Ki-67||ICFC,ICC|
|Alexa Fluor® 488 anti-human Ki-67||Ki-67||ICFC,ICC|
|Alexa Fluor® 647 anti-human Ki-67||Ki-67||ICFC,ICC|
|Pacific Blue™ anti-human Ki-67||Ki-67||ICFC|
|APC anti-human Ki-67||Ki-67||ICFC|
|Brilliant Violet 711™ anti-human Ki-67||Ki-67||ICFC|
|PerCP/Cyanine5.5 anti-human Ki-67||Ki-67||ICFC|
|Brilliant Violet 605™ anti-human Ki-67||Ki-67||ICFC|
|PE/Cyanine7 anti-human Ki-67||Ki-67||ICFC|
|Purified anti-human Ki-67 (Maxpar® Ready)||Ki-67||ICFC,CyTOF®|
|Alexa Fluor® 594 anti-human Ki-67||Ki-67||ICC|
|Alexa Fluor® 700 anti-human Ki-67||Ki-67||ICFC|
|PE/Dazzle™ 594 anti-human Ki-67||Ki-67||ICFC|
|Brilliant Violet 750™ anti-human Ki-67||Ki-67||ICFC|
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Brilliant Violet 510™ anti-human Ki-67
Purified anti-human Ki-67
PE anti-human Ki-67
Brilliant Violet 421™ anti-human Ki-67
Alexa Fluor® 488 anti-human Ki-67
Alexa Fluor® 647 anti-human Ki-67
Pacific Blue™ anti-human Ki-67
APC anti-human Ki-67
Brilliant Violet 711™ anti-human Ki-67
PerCP/Cyanine5.5 anti-human Ki-67
Brilliant Violet 605™ anti-human Ki-67
PE/Cyanine7 anti-human Ki-67
Purified anti-human Ki-67 (Maxpar® Ready)
Alexa Fluor® 594 anti-human Ki-67
Alexa Fluor® 700 anti-human Ki-67
PE/Dazzle™ 594 anti-human Ki-67
Brilliant Violet 750™ anti-human Ki-67