Alexa Fluor® 647 anti-human CD79a (Igα) Antibody

Pricing & Availability
Clone
HM47 (See other available formats)
Regulatory Status
RUO
Workshop
V cB017
Other Names
Mb-1, Iga, CD79
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A-HM47_A647_CD79a_Antibody_1_050619
Human peripheral blood lymphocytes surface stained with CD19 Alexa Fluor® 488, then fixed, permeabilized, and intracellularly stained with CD79a (Igα) (clone HM47) Alexa Fluor® 647 (left) or Mouse IgG1, κ isotype control (right) Alexa Fluor® 647.
  • A-HM47_A647_CD79a_Antibody_1_050619
    Human peripheral blood lymphocytes surface stained with CD19 Alexa Fluor® 488, then fixed, permeabilized, and intracellularly stained with CD79a (Igα) (clone HM47) Alexa Fluor® 647 (left) or Mouse IgG1, κ isotype control (right) Alexa Fluor® 647.
  • B-HM47_A647_CD79a_Antibody_052218
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10 mM, pH 6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 anti-human CD8a (clone C8/144B, green) antibody and Alexa Fluor® 647 anti-human CD79a (clone HM47, red) antibody over night at 4°C. The nuclei were counter stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
333515 25 µg £92.00
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333516 100 µg £215.00
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Description

CD79a is a 47 kD type I integral membrane protein, also known as mb-1 or Iga. It is a member of the Ig superfamily and disulphide-associated with CD79b (B29). The interaction of CD79a/CD79b heterodimer with B cell suface Ig forms B cell antigen complex. CD79a is expressed in B cells from early pre-B to plasma cell stage. It has been shown that CD79a is also weakly expressed in some precursors of T- and myeloid cells. CD79 mediates the transport of IgM to B cell surface and transduces signals initiated by BCR aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_2734330 (BioLegend Cat. No. 333515)
AB_2734331 (BioLegend Cat. No. 333516)

Antigen Details

Structure
47 kD, type I integral protein, Ig superfamily, CD79a/CD79b heterodimer interacts with B cell membrane Ig.
Distribution

B cells (from early pre-B to plasma cell stage), some T and myeloid cell precursors

Function
In association with CD79b and B cell membrane Ig to form B cell antigen receptor complex
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS
2. Astsaturov IA, et al. 1996. Leukemia 10:769
3. Mson DY, et al. 1995 Blood 86:1453
4. Hashimoto M, et al. 2002. J. Pathol. 197:341

Gene ID
973 View all products for this Gene ID
UniProt
View information about CD79a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/06/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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