Alexa Fluor® 647 anti-human CD285 (TLR5) Antibody

Pricing & Availability
Clone
S16021I (See other available formats)
Other Names
SLE1, TIL3, SLEB1, MELIOS, CD285
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
S16021I_A647_CD285_Antibody_100819.png
Human peripheral blood monocytes were stained with CD14 FITC and CD285 (TLR5) (clone S16021I) Alexa Fluor® 647 (left) or Mouse IgG2a, κ Alexa Fluor® 647 isotype control (right).
  • S16021I_A647_CD285_Antibody_100819.png
    Human peripheral blood monocytes were stained with CD14 FITC and CD285 (TLR5) (clone S16021I) Alexa Fluor® 647 (left) or Mouse IgG2a, κ Alexa Fluor® 647 isotype control (right).
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
394505 25 tests £96
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394506 100 tests £219
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Description

CD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TLR5 transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)

Antigen Details

Structure
Type 1 transmembrane protein, 98 kD
Distribution

Neutrophils, classical monocytes, dendritic cells, mucosal epithelium

Function
Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
Ligand/Receptor
flagellin
Cell Type
Dendritic cells, Epithelial cells, Monocytes, Neutrophils
Biology Area
Immunology
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References
  1. Hayashi F, et al. 2001. Nature. 410:1099
  2. Gewirtz AT, et al. 2001. J Immunol. 167:1882
  3. Smith KD, et al. 2002. Curr Top Microbiol Immunol. 270:93
  4. Bambou JC, et al. 2004. J Biol Chem. 279:42984
  5. Sebastiani G, et al. 2000. Genomics. 64:230
  6. Muzio M, et al. 2000. Biochem Soc Trans. 28:563
Gene ID
7100 View all products for this Gene ID
UniProt
View information about CD285 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/08/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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