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Alexa Fluor® 647 anti-GFAP Antibody

Pricing & Availability
Clone
2E1.E9 (See other available formats)
Regulatory Status
RUO
Other Names
Glial fibrillary acid protein
Isotype
Mouse IgG2b
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Product Citations
publications
a-2E1-E9_A647_GFAP_Antibody_1_022620
C57BL/6 mouse frozen cerebellum section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 2.5 µg/mL of GFAP (clone 2E1.E9) Alexa Fluor® 647 (magenta) overnight at 4°C. Nuclei were counterstained with DAPI (green). The image was captured by 10X objective. Scale Bar: 50 µm
  • a-2E1-E9_A647_GFAP_Antibody_1_022620
    C57BL/6 mouse frozen cerebellum section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 2.5 µg/mL of GFAP (clone 2E1.E9) Alexa Fluor® 647 (magenta) overnight at 4°C. Nuclei were counterstained with DAPI (green). The image was captured by 10X objective. Scale Bar: 50 µm
  • b-2E1dotE9_A647_GFAP_Antibody_IHC_2_091117
    Human paraffin-embedded cerebellum tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (1.0M, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10µg/mL of Alexa Fluor® 647 anti-GFAP Antibody (Clone 2E1.E9, red) and Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) Antibody (Clone AA10, green) antibody overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • c-2E1dotE9_A647_GFAP_Antibody_IHC_3_091117
    Human paraffin-embedded cerebellum tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (1.0M, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10µg/mL of Alexa Fluor® 647 anti-GFAP Antibody (Clone 2E1.E9, red) and Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) Antibody (Clone AA10, magenta) antibody overnight at 4°C. Nuclei were counterstained with DAPI (green). The image was scanned with a 10X objective and stitched with MetaMorph® software.
Compare all formats See Alexa Fluor® 647 spectral data
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644706 100 µg £221
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Description

GFAP is expressed exclusively in astrocytes in the central nervous system. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. This antibody does not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. More than 50 GFAP mutations have been identified to be associated with the Alexander disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Bovine spinal cord homogenate
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. McLendon RE and Bigner DD. 1994. Brain Pathol. 4:221.
  2. Liu W, et al. 2011. Proteomics 11:3556. (FC) PubMed
Product Citations
  1. Tyler CR, et al. 2018. Toxicol Sci. 163:123. PubMed
  2. Rocktäschel P, et al. 2019. Epilepsy Behav. 101:106581. PubMed
RRID
AB_2566110 (BioLegend Cat. No. 644706)

Antigen Details

Structure
Around 50 kD
Distribution

Only expressed in astrocytes in the central nervous system.

Function
Together with other proteins to form intermediate filaments which supports astroglial cells. Astroglial cells support and nourish cells in the brain and spinal cord. If brain cells are injured through trauma or disease, astroglial cells react by rapidly prodcing more GFAP protein.
Cell Type
Astrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/26/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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