Alexa Fluor® 594 Streptavidin

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Other Names
Streptavidin-Alexa Fluor® 594, Streptavidin-AF594
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Product Citations
publications
1_SAV_AF594_Streptavidin_Antibody_IF_042314
Mouse embryonic fibroblast NIH/3T3 cell line was fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with anti-mouse/human CD44 biotinylated antibody (clone IM7), followed by Streptavidin Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). This image was captured with a 20X objective.
  • 1_SAV_AF594_Streptavidin_Antibody_IF_042314
    Mouse embryonic fibroblast NIH/3T3 cell line was fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with anti-mouse/human CD44 biotinylated antibody (clone IM7), followed by Streptavidin Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). This image was captured with a 20X objective.
  • 2_A594_Streptavidin_IHC-F_070617
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of GL7 (clone GL7) purified, CD3 (clone 17A2) Alexa Fluor® 647 (green) and B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgM (clone MRM-47) Biotin for 2 hours, followed by 2.5 µg/ml of Streptavidin Alexa Fluor® 594 (red) for 2 hours at room temperature. The image was captured by 10X objective.
See Alexa Fluor® 594 spectral data See high resolution IF/IHC-F data...
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405240 100 µg £151
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Alexa Fluor® 594 is useful for detecting biotinylated antibodies.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin was conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-Alexa Fluor® 594 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IF - Quality tested
IHC-F - Validated 

Recommended Usage

This streptavidin product is quality control tested by immunofluorescence in microscopy. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Alexa Fluor® 594 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 2.5-5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® is a trademark of Life Technologies Corporation.

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Application Notes

Streptavidin-Alexa Fluor® 594 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-Alexa Fluor® 594 is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245.
  2. Nishimoto KP,et al. 2008. J. Immunol. 181:4010. PubMed
  3. Niki T, et al. 2009. J. Biol. Chem. 284:32344. PubMed
  4. Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed
  5. Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed
  6. Yamakawa N, et al. 2012. Int Immunol. PubMed
  7. Shibata T, et al. 2012. Int Immunol. 24:613. PubMed
  8. Baccala R, et al. 2012. J. Immunol. 189:5976. PubMed
  9. Grevers LC, et al. 2013. Ann Reheum Dis. 72:278. PubMed
  10. Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed
  11. Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed
  12. Reading JL, 2013. J. immunol. 190:4542. Pubmed
  13. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed
  14. Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed
Product Citations
  1. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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