Alexa Fluor® 594 Goat anti-hamster (Armenian) IgG Antibody

Pricing & Availability
Clone
Poly4055 (See other available formats)
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly4055_AF594_GoatAntiHamsterIgG_Antibody_IHCF_082316
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 5 µg/ml of purified CD3ε (clone 145-2C11) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 Goat anti-hamster (Armenian) IgG (red) and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) for two hours at room temperature. The image was captured with a 10X objective.
  • Poly4055_AF594_GoatAntiHamsterIgG_Antibody_IHCF_082316
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 5 µg/ml of purified CD3ε (clone 145-2C11) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 Goat anti-hamster (Armenian) IgG (red) and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) for two hours at room temperature. The image was captured with a 10X objective.
See Alexa Fluor® 594 spectral data See high resolution IHC-F data...
Cat # Size Price Quantity Avail. Save
405512 100 µg £94
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Description

This polyclonal antibody reacts with the heavy chains of IgG and the light chains common to most immunoglobulins from Armenian hamster. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with mouse, rat, human, bovine, and rabbit immunoglobulins, but may cross-react with Armenian hamster IgM and Syrian hamster immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Armenian Hamster
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.


Alexa Fluor® is a trademark of Life Technologies Corporation.

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Application Notes

This conjugated goat anti-hamster IgG antibody is useful for immunofluorescent staining with flow cytometric analysis or microscopy.

Product Citations
  1. Liu M, et al. 2019. Front Immunol. 10:477. PubMed
Publication Library
RRID
AB_2629746 (BioLegend Cat. No. 405512)

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Go To Top Version: 1    Revision Date: 02/08/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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