Alexa Fluor® 594 anti-Nur77 Antibody

Pricing & Availability
Clone
1E10A15 (See other available formats)
Other Names
Nuclear receptor subfamily 4, group A, member 1 (NR4A1), GFRP, HMR, Testicular receptor 3 (TR3)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1E10A15_A594_Nur77_ICC_033114
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-Nur77 (clone 1E10A15) Alexa Fluor® 594 (red) in blocking buffer, overnight at 4°C, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • 1E10A15_A594_Nur77_ICC_033114
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-Nur77 (clone 1E10A15) Alexa Fluor® 594 (red) in blocking buffer, overnight at 4°C, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
653504 100 µg £202
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Description

Nur77, a transcription factor belonging to a superfamily of orphan nuclear receptors, has been shown to activate a subset of genes containing NGFI-B response element (NBRE). Nur77 expression is upregulated in response to stimuli of numerous growth factors, mitogens, and inflammatory substances, indicating that Nur77 has regulatory roles in cell growth and inflammation. Nur77 has also been shown to participate in programmed cell death through mitochondrial targeting and interaction with Bcl-2. In the endocrine system, Nur77 has been reported to participate in regulating production of steroidogenic enzymes. Nur77 and NOR-1 double knockout mice develop acute myeloid leukemia, suggesting that Nur77 plays a role in regulating differentiation of myeloid progenitor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human Nur77 recombinant protein (1-266 aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2563321 (BioLegend Cat. No. 653504)

Antigen Details

Structure
64 kD protein containing a DNA-binding domain, two zinc finger domain, and a potential ligand-binding domain
Distribution

Nucleus

Function
Nur77 is a transcription factor that mediates various physiological stimuli, such as growth factors, hormones, inflammatory, and energy metabolic signals. Nur77 plays roles in many important biological processes including cell growth, differentiation, and apoptosis.
Interaction
Interacts with GADD45GIP1 and Bcl-2.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Nuclear Markers, Steroid Receptors/Nuclear Receptors
Antigen References

1. Nakai A, et al. 1990. Mol. Endocrinol. 4:1438.
2. Han YH, et al. 2006. Oncogene 25:2974.
3. Li M, et al. 2010. Mol. Cell. Endocrinol. 319:39.
4. Pearen MA, et al. 2010. Mol. Endocrinol. 24:1891.
5. Moll UM, et al. 2006. Oncogene 25:4725.
6. Fassett MS, et al. 2012. Proc. Natl. Acad. Sci. USA 109:3891.

Gene ID
3164 View all products for this Gene ID
UniProt
View information about Nur77 on UniProt.org

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Go To Top Version: 1    Revision Date: 04/15/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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