Alexa Fluor® 594 anti-mouse Podoplanin Antibody

Pricing & Availability
Clone
PMab-1 (See other available formats)
Other Names
T1a, gp38, stromal cell marker
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
PMab-1_A594_Podoplanin_Antibody_1_012319
C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/ml of Alexa Fluor® 488 anti-mouse CD326/EpCAM (clone G8.8, green), 10 µg/ml of Alexa Fluor® 594 anti-mouse podoplanin (clone PMab-1, red) at 4°C overnight. Nuclei was counter stained with DAPI (blue). The image was scanned with a 10X object and stitched with MetaMorph® software.
  • PMab-1_A594_Podoplanin_Antibody_1_012319
    C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/ml of Alexa Fluor® 488 anti-mouse CD326/EpCAM (clone G8.8, green), 10 µg/ml of Alexa Fluor® 594 anti-mouse podoplanin (clone PMab-1, red) at 4°C overnight. Nuclei was counter stained with DAPI (blue). The image was scanned with a 10X object and stitched with MetaMorph® software.
  • PMab-1_A594_Podoplanin_Antibody_2_050819
    Formalin-fixed, 300 micron-thick mouse kidney section was blocked, permeabilized and stained overnight with CD31 (clone MEC13.3) Alexa Fluor® 647 (red) at 5 µg/mL, Tubulin Beta 3 (TUBB3)(clone AA10) Alexa Fluor® 488 (green) at 2.5 µg/mL, and Podoplanin (clone Pmab-1) Alexa Fluor® 594 (blue) at 5 µg/mL, optically cleared, then analyzed at 230 µm imaging depth on a confocal microscope. Watch the video.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
156205 25 µg £99
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156206 100 µg £202
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Description

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Peptide (38-51 amino acids of mouse podoplanin)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
3-D IHC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application References

(PubMed link indicates BioLegend citation)
  1. Yamada S, et al. 2017. Monoclon Antib Immunodiagn Immunother. 36(2):77-79.
RRID
AB_2800698 (BioLegend Cat. No. 156205)
AB_2819915 (BioLegend Cat. No. 156206)

Antigen Details

Structure
43 kD glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution

Expressed on epithelial and mesothelial cells.

Function
Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation.
Interaction
Interacts with CLEC1B; the interaction is independent of CLEC1B glycosylation and activates CLEC1B; the interaction is dependent of sialic acid on O-glycans.
Ligand/Receptor
CLEC1B
Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Farr A, et al. 1992. J Histochem Cytochem. 40:651.
  2. Schacht V, et al. 2005. Am J Pathol. 166:913.
Gene ID
14726 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05/08/2019

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