Alexa Fluor® 594 anti-mouse IgG1 Antibody

Pricing & Availability
Clone
RMG1-1 (See other available formats)
Other Names
Immunoglobulin G1
Isotype
Rat IgG
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Product Citations
publications
RMG1-1_A594_IgG1_Antibody_102419.png
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Critic buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 5 µg/mL of purified anti-human CD8a (clone C8/144B) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with rat anti-mouse IgG1 (clone RMG1-1) Alexa Fluor® 594 (red) and anti-human CD19 (clone A17136C) Alexa Fluor® 647 (green) for an hour at room temperature. The nuclei were counter staining with DAPI (blue). The image was scanned with a 10X objective and stitched with Metamorph® software.
  • RMG1-1_A594_IgG1_Antibody_102419.png
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Critic buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 5 µg/mL of purified anti-human CD8a (clone C8/144B) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with rat anti-mouse IgG1 (clone RMG1-1) Alexa Fluor® 594 (red) and anti-human CD19 (clone A17136C) Alexa Fluor® 647 (green) for an hour at room temperature. The nuclei were counter staining with DAPI (blue). The image was scanned with a 10X objective and stitched with Metamorph® software.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
406634 100 µg £109
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Description

The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® is a trademark of Life Technologies Corporation.

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Application References

(PubMed link indicates BioLegend citation)
  1. Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed
  2. Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed

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Go To Top Version: 1    Revision Date: 10/24/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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