Alexa Fluor® 594 anti-mouse CD272 (BTLA) Antibody

Pricing & Availability
Clone
6A6 (See other available formats)
Regulatory Status
RUO
Other Names
BTLA, B and T lymphocyte attenuator
Isotype
Armenian hamster IgG
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Product Citations
publications
6A6_A594_CD272_Antibody_1_111519
C57BL/6 frozen mouse spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 2.5 µg/mL of Alexa Fluor® 594 anti-mouse CD272 (clone 6A6) (red), 5 µg/mL of Alexa Fluor® 647 anti-mouse CD3 (clone 17A2) (green), and 5 µg/mL of Alexa Fluor® 488 anti-mouse/human CD45R/B220 (clone RA3-6B2) (blue) at 4°C overnight. The image was captured with a 10X objective.
  • 6A6_A594_CD272_Antibody_1_111519
    C57BL/6 frozen mouse spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for one hour at room temperature. Then the section was stained with 2.5 µg/mL of Alexa Fluor® 594 anti-mouse CD272 (clone 6A6) (red), 5 µg/mL of Alexa Fluor® 647 anti-mouse CD3 (clone 17A2) (green), and 5 µg/mL of Alexa Fluor® 488 anti-mouse/human CD45R/B220 (clone RA3-6B2) (blue) at 4°C overnight. The image was captured with a 10X objective.
See Alexa Fluor® 594 spectral data
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139111 100 µg £222.00
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Description

CD272, also known as B and T lymphocyte attenuator (BTLA), is an Ig superfamily co-inhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.  BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand herpes virus entry mediator (HVEM) is critical for negatively regulating immune response.  The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells.  BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo.  Tolerant T cells have significantly higher expression of BTLA compared with effectors and naïve T cells.  BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity.  It was reported that BTLA may regulate T cell function by binding to B7-H4, but further studies are needed to confirm.  The existence of three distinct BTLA alleles has been reported.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
C57BL/6 BTLA Ig domain protein in CFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunohistochemistry, a concentration range of 2.5 - 10 μg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1,2 and blocking3,5 of OT-I T cell responses in vitro and incidence of cerebral malaria in vivo caused by P. berghei ANKA (PbA) infection. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 139104). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 139117 - 139122) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Clone 6A6 antibody reacts only with the C57BL/6 allele of BTLA but not the BALB/c allele of BTLA.

Application References

(PubMed link indicates BioLegend citation)
  1. Hurchla MA, et al. 2005. J. Immunol. 174:337. (IP FC)
  2. Sedy JR, et al. 2005. Nat. Immunol. 6:90. (IP)
  3. Lepenies B, et al. 2007. J. Immunol. 179:4093. (Block)
  4. Tao R, et al. 2005. J. Immunol. 175:5774. (FC)
  5. Albring J, et al. 2010. J. Exp. Med. 207:2551. (Block)
Product Citations
RRID
AB_2650850 (BioLegend Cat. No. 139111)

Antigen Details

Structure
An Ig superfamily co-inhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.
Distribution

BTLA is expressed on a wide number of lymphocytes in mice. It is most highly expressed on B cells, followed by CD4+ T cells, lower expression on CD8+ T cells, macrophages, dendritic cells, NKT cells, and NK cells.

Function
BTLA functions as a negative regulator of T cell activation and proliferation, attenuate B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).
Ligand/Receptor
HVEM.
Cell Type
B cells, Dendritic cells, Lymphocytes, NKT cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Liu X, et al. 2009. J Immunol. 182:4516.
2. Miller ML, et al. 2009. J Immunol. 183:32.
3. Sun Y, et al. 2009. J Immunol. 183:1946.
4. Vendel AC, et al. 2009. J Immunol. 182:1509.
5. Watanabe N, et al. 2003. Nat Immunol. 4:670.

Gene ID
208154 View all products for this Gene ID
UniProt
View information about CD272 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/13/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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