Alexa Fluor® 594 anti-IGF2BP2 Antibody

Pricing & Availability
Clone
O93C8 (See other available formats)
Regulatory Status
RUO
Other Names
IMP2, Hepatocellular Carcinoma Autoantigen P62, VICKZ2, IGF-II mRNA binding protein, Insulin-like growth factor 2, P62
Isotype
Mouse IgG1
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Product Citations
publications
O93C8_A594_IGF2BP2_Antibody_1_110818
HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes and then were permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. The cells were then intracellularly stained with 2.5 µg/ml Alexa Fluor® 594 anti-IGF2BP2 antibody (clone O93C8) (red) in a blocking buffer overnight at 4°C, followed by Alexa Fluor® 488 phalloidin (green) staining for 20 minutes at room temperature. Nuclei were counterstained with DAPI (blue).
  • O93C8_A594_IGF2BP2_Antibody_1_110818
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for ten minutes and then were permeabilized with 0.5% Triton X-100 for five minutes followed by blocking with 5% FBS for 30 minutes. The cells were then intracellularly stained with 2.5 µg/ml Alexa Fluor® 594 anti-IGF2BP2 antibody (clone O93C8) (red) in a blocking buffer overnight at 4°C, followed by Alexa Fluor® 488 phalloidin (green) staining for 20 minutes at room temperature. Nuclei were counterstained with DAPI (blue).
See Alexa Fluor® 594 spectral data
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679604 100 µg £236.00
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Description

IGF2BP2 belongs to the family of Insulin-like growth factor-II mRNA binding proteins (IMP1, IMP2, and IMP3). Members in this family contain four hnRNP K homology (KH) domains and two RNA recognition motifs (RRM) domains. They are involved in the RNA cytoplasmic localization, stability, and translation. Recent studies suggest that IGF2BP2 and IGF2BP1 bind to target mRNAs via all four KH domains. Studies showed that the expression of IGF2BP2 is associated with liposarcoma, liver cancer, and endometrial adenocarcinomas. Genome-wide association studies have also identified IGF2BP2 as one of the several factors for developing type 2 diabetes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human IGF2BP2 protein (NP_006539) produced in HEK 293T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2572061 (BioLegend Cat. No. 679604)

Antigen Details

Structure
Canonical sequence contains 599 amino acids with a predicted molecular weight of 66.1 kD. Five isoforms with a molecular weight ranging from 54.7 kD to 61.8 kD.
Distribution

Nucleus, cytoplasm, and the cytoskeleton. It is expressed in oocytes, granulosa cells, Leydig cells, spermatogonia, but is expressed weaker in the lymph nodes, lungs, liver, kidney, heart muscles, spleen, and testes.

Function
IGF2BP2 binds to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulates IGF2 translation. It also modulates the cytoplasmic trafficking, turnover, and translation of various target mRNAs.
Interaction
Interacts with HNRPD, IGF2BP1, IGF2BP3, ELAVL1, DHX9, and HNRNPU.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Wachter K, et al. 2013. Biol. Chem. 394:1077.
2. Zhao Y, et al. 2012. DNA Cell Biol. 31:713.
3. Bell JL, et al. 2013. Cell Mol. Life Sci. 15:2657.
4. Ng MC, et al. 2008. Diabetes 57:2226.
5. Scott LJ, et al. 2007. Science 316:1341.
6. Nielsen FC, et al. 2002. J. Cell Sci. 115:2087.
7. Nielsen J, et al. 1999. Mol. Cell Biol. 2:1262.

Gene ID
10644 View all products for this Gene ID
UniProt
View information about IGF2BP2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/10/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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