Alexa Fluor® 594 anti-human VEGFR1 Antibody

Pricing & Availability
Clone
A16083C (See other available formats)
Other Names
Fms-related tyrosine kinase 1, oncogene FLT, FLT, FLT-1, VEGFR1, VEGFR-1, VEGFR
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
A16083C_A594_VEGFR1_Antibody_100118_updated
Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human VEGFR1 (clone A16083C) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • A16083C_A594_VEGFR1_Antibody_100118_updated
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human VEGFR1 (clone A16083C) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
394903 25 µg £91
Check Availability


Need larger quantities of this item?
Request Bulk Quote
394904 100 µg £198
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

VEGF-R1, also known as Fms-related tyrosine kinase 1 (FLT-1), is type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases.  Human VEGF-R1 is mainly expressed by endothelial cells, which plays central role in vasculogenesis, angiogenesis and lymphaniogenesis. VEGFR-1 is the only VEGFR present on the surface of monocytes, and it mediates a chemotactic response to VEGF-A and tissue factor induction. It also reported on placental trophoblast cells, osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells. VEGFR1  is regulated by PEDF, which effects its intracellular proteolysis rate. sVEGFR1 regulates the availability of VEGFA and is produced in the placenta under hypoxic conditions. The ligands of VEGF-R1 include VEGF-A, VEGF-B, and PIGF splice isoforms.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human VEGFR1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
RRID
AB_2783307 (BioLegend Cat. No. 394903)
AB_2783308 (BioLegend Cat. No. 394904)

Antigen Details

Structure
Cytokine receptor
Distribution

Endothelial cells, placental trophoblast cells, peripheral blood monocytes and macrophages

Function
Angiognesis and vasculogenesis
Cell Type
Endothelial cells, Macrophages, Monocytes
Antigen References
  1. Kendall RL, et al. 1996. Biochem Biophys Res Commun. 226:324.
  2. Cai J, et al. 2006. J Biol Chem. 281:3604.
  3. Ambati BK, et al. 2006. Nature. 443:993.
  4. Heydarian M, et al. 2009. Placenta. 30:250.
  5. Thomas CP, et al. 2009. J Clin Endocrinol Metab. 94:2524.
  6. Wu, et al. 2010. J Cell Mol Med. 14:528.
  7. Cai J, et al. 2011. PLoS One. 6:e18076.
Gene ID
2321 View all products for this Gene ID
UniProt
View information about VEGFR1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/03/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account