- A15102C (See other available formats)
- Other Names
- Lectin, galactoside-binding, soluble, 4 (LGALS4), GAL4, L36LBP, Galectin
- Mouse IgG2b, κ
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- Product Citations
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Galectin-4, also known as LGALS4, is a member of galectin family, a family of conserved animal lectins, which are characterized by their affinity for poly-N-acetyllactosamine-enriched glycoconjugates and sequence similarities in the carbohydrate recognition domain (CRD). Galectin-4 was initially identified in gastrointestinal mucosa including oral mucosa and esophagus. RNAse protection assays revealed that it is not expressed in the brain, kidney, skeletal muscle, heart, liver, and lung tissues. Similar to other galectins, galectin-4 does not have a signal peptide and is synthesized as a cytosolic protein, but it can be secreted. Galectin-4 controls intestinal inflammation and it binds to activated T cells at the CD3 region, which results in a potent inhibition of cell cycle progression and induction of antigen-induced cell death. Galectin-4 induces T cell death through the caspase-independent calcium-calpain signaling pathway. Galectin-4 is downregulated in colon cancer tissue and is absent in invasive carcinomas. Nevertheless, high levels in serum are detected in patients with colon and breast cancer. In contrast, it is considerably upregulated in pancreatic adenocarcinomas, hepatocellular carcinomas, and gastric cancer.Product Details
- Antibody Type
- Host Species
- Recombinant human Galectin-4
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-P - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Contains at least two distinct carbohydrate recognising domains (CRD).
It is expressed in oral mucosa, esophagus, gastrointestinal epithelium, and intestinal epithelial cell lines.
- Controls intestinal inflammation, intestinal epithelial wound healing, protein apical trafficking, and stabilizes adherens junctions and lipid rafts in the microvillus membrane.
- T cells and macrophages.
- Galectin-4 binds to glycosphingolipids with 3-O-sulfated Gal residues and carcinoembryonic antigens.
- Cell Type
- Epithelial cells
- Biology Area
- Cancer Biomarkers, Immunology
- Antigen References
1. Rabinovich GA, et al. 2002. J Leuko Biol. 71:741.
2. Huflejt ME, Leffler H. 2004. Glycoconj J. 20:247.
3. Ideo H, et al. 2005. J Biol Chem. 6:4730-7.
4. Markova V, et al. 2006. Int J Mol Med. 18:65.
5. Paclik D, et al. 2008. Inflamm Bowel Dis. 14:1366.
6. Paclik D, et al. 2008. PLoS One 3(7):e2629.
7. Belo A, et al. 2013. PLoS One 8(6):e65957.
8. Houzelstein D, et al. 2013. J Histochem Cytochem. 61:348.
9. Viguier M, et al. 2014. Tissue Barriers 2:e29103.
10. Cai Z, et al. 2014. Cancer Sci. 105:1510.
- Gene ID
- 3960 View all products for this Gene ID
- View information about Galectin-4 on UniProt.org
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