Alexa Fluor® 594 anti-human CD107b (LAMP-2) Antibody

Pricing & Availability
Clone
H4B4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
LAMPb, LGP110
Isotype
Mouse IgG1, κ
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Product Citations
publications
H4B4_A594_CD107b_Antibody_101717
Human paraffin-embedded prostate tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris Buffered 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 anti-human CD107b (clone H4B4, red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • H4B4_A594_CD107b_Antibody_101717
    Human paraffin-embedded prostate tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris Buffered 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 anti-human CD107b (clone H4B4, red) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Check Availability Save
354309 25 µg £92.00
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354310 100 µg £222.00
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Description

CD107b, also known as LAMP-2, is a 105 kD, highly gylcosylated, type I transmembrane protein. CD107b is expressed in lysosomal/endosomal membranes in nearly all cells, and on the surface of activated platelets, activated lymphocytes and some tumor cell lines.  LAMP-2 is known to have roles in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Adult human adherent spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

 

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen glomeruli2 and immunofluorescent staining of  neutrophils2,3.

This antibody is specific to human LAMP-2. Positive control: Hela cells; LAMP-2 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References

(PubMed link indicates BioLegend citation)
  1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
  2. Kain R, et al. 2008. Nat. Med. 14:1088. (IF, IHC)
  3. Roark EA, et al. 2008. PLoS ONE 3:e3538. (IF)
RRID
AB_2721377 (BioLegend Cat. No. 354309)
AB_2721378 (BioLegend Cat. No. 354310)

Antigen Details

Structure
LAMP-2 is a 410 amino acid protein with a molecular mass of 45 kD.
Distribution

Lysosomal/endosomal membranes in nearly all cells; surface of activated platelets, activated lymphocytes and some tumor cell lines

Function
Adhesion, cellular homeostatis, including autophagocytosis, antigen presentation
Cell Type
B cells, Lymphocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
2. Kain R, et al. 2008. Nat. Med. 14:1088.
3. Roark EA, et al. 2008. PLoS ONE 3:e3538.

Gene ID
3920 View all products for this Gene ID
UniProt
View information about CD107b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 10/17/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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