Alexa Fluor® 594 anti-Histone H3 Phospho (Ser10) Antibody

Pricing & Availability
Clone
11D8 (See other available formats)
Other Names
Histone-H3, H3
Isotype
Mouse IgG2b, κ
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11D8_A594_Histone_H3_Phospho_Antibody_031119.png
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 594 Mouse IgG2b, κ Isotype Ctrl Antibody (Negative, Cat. No. 400362) or (B-D) Alexa Fluor® 594 Histone H3 Phospho (Ser10) Antibody (Clone 11D8) overnight at 4°C. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/400. Concentrations for (A-B) is 2 µg/ml (1:250 dilution), (C) is 0.5 µg/ml (1:1000 dilution) and (D) is 0.1 µg/ml (1:5000 dilution).
  • 11D8_A594_Histone_H3_Phospho_Antibody_031119.png
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 594 Mouse IgG2b, κ Isotype Ctrl Antibody (Negative, Cat. No. 400362) or (B-D) Alexa Fluor® 594 Histone H3 Phospho (Ser10) Antibody (Clone 11D8) overnight at 4°C. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/400. Concentrations for (A-B) is 2 µg/ml (1:250 dilution), (C) is 0.5 µg/ml (1:1000 dilution) and (D) is 0.1 µg/ml (1:5000 dilution).
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
650809 25 µg £91
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650810 100 µg £212
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Description

Histone H3 is phosphorylated at serine 10 during mitosis and is found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Modified synthetic peptide conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2 - 5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residue and has been shown to be useful for Western blotting.

RRID
AB_2801122 (BioLegend Cat. No. 650809)
AB_2801123 (BioLegend Cat. No. 650810)

Antigen Details

Structure
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution

Nucleus

Function
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones, therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication, and chromosomal stability.
Interaction
Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.
4. Hans F, et al. 2001. Oncogene 20:3021.

Regulation
H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Gene ID
8290 View all products for this Gene ID
UniProt
View information about Histone H3 Phospho on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03/11/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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