Alexa Fluor® 594 anti-GluR2 Antibody

Pricing & Availability
Clone
L21/32 (See other available formats)
Other Names
GluA2/GluR2 glutamate receptor, Glutamate receptor 2, GluR2, gluR-2, glutamate receptor B, AMPA-selective glutamate receptor 2
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
L21-32_A594_GluR2_Antibody_012519.png
IHC staining of Alexa Fluor® 594 anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 60X objective. Scale Bar: 20 µm
  • L21-32_A594_GluR2_Antibody_012519.png
    IHC staining of Alexa Fluor® 594 anti-GluR2 antibody (clone L21/32) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 60X objective. Scale Bar: 20 µm
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
810503 25 µg £74
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810504 100 µg £121
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Description

Glutamate receptor 2 (GluR2), also known as glutamate receptor B, is a subunit of AMPA receptors (AMPARs) involved in fast excitatory synaptic transmission. GluR2 regulates the permeability of an AMPAR to sodium, potassium, and calcium, and the absence of GluR2 allows AMPARs to be blocked by polyamines.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 834-883 of rat GluA2/GluR2 glutamate receptor.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P). It was subsequently purified using protein G. It does not cross-react with GluA1/GluR1, GluA3/GluR3, or GluA4/GluR4.

Additional Product Notes

The reactivity of Alexa Fluor® 594 anti-GluR2 antibody was validated in FFPE mouse brain tissue.

Antigen Details

Structure
Expected Molecular Weight: 90 kD
Cell Type
Glutamatergic Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
29627 View all products for this Gene ID
UniProt
View information about GluA2/GluR2 glutamate receptor on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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