Alexa Fluor® 594 anti-eIF4A1 Antibody

Pricing & Availability
Clone
W17130C (See other available formats)
Other Names
EIF4A1, Eukaryotic Translation Initiation Factor 4A1
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
W17130C_A594_elF4A1_Antibody_051019.png
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl Antibody (Negative, Cat. No. 400555) or (B-D) Alexa Fluor® 594 anti EIF4A1/DDX2A Antibody (Clone W17130C) overnight at 4°C. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/20. Concentrations for (A-B) is 0.4 µg/mL, (C) is 0.2 µg/mL and (D) is 0.1 µg/mL.
  • W17130C_A594_elF4A1_Antibody_051019.png
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl Antibody (Negative, Cat. No. 400555) or (B-D) Alexa Fluor® 594 anti EIF4A1/DDX2A Antibody (Clone W17130C) overnight at 4°C. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/20. Concentrations for (A-B) is 0.4 µg/mL, (C) is 0.2 µg/mL and (D) is 0.1 µg/mL.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
666203 25 µg £91
Check Availability


Need larger quantities of this item?
Request Bulk Quote
666204 100 µg £212
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

EIF4A1 is an ATP-dependent RNA helicase. It can form the eIF4F complex, which is involved in translation initiation by binding with the 5' m7 GTP cap of the mRNA. It could unwind mRNA secondary structure at the 5’ UTR region and lead to the small ribosomal subunit binding and followed scanning of the initiator codon.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Hamster
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human EIF4A1 Peptide (3-19 a.a.) conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 0.4 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Antigen Details

Distribution

Cytoplasm

Function
Translation initiation
Interaction
eIF4 complex
Biology Area
Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Synthesis, Signal Transduction
Gene ID
1973 View all products for this Gene ID
UniProt
View information about eIF4A1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/10/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account