Alexa Fluor® 488 anti-Synapsin I/II/III Antibody

Pricing & Availability
Clone
A17080A (See other available formats)
Other Names
SYN, Synapsin, Synapsin I, Synapsin II, Synapsin III
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
A17080A_A488_Synapsin_I-II-III_Antibody_1_081419
IHC staining of Alexa Fluor® 488 anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • A17080A_A488_Synapsin_I-II-III_Antibody_1_081419
    IHC staining of Alexa Fluor® 488 anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
  • A17080A_A488_Synapsin_I-II-III_Antibody_2_081419
    IHC staining of Alexa Fluor® 488 anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 10X objective. Scale Bar: 100 µm
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
853711 25 µg £97
Check Availability


Need larger quantities of this item?
Request Bulk Quote
853712 100 µg £202
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Synapsins are a family of proteins involved in neurotransmitter release at the chemical synapses. Synapsins anchor synaptic vesicles to the cytoskeletal structures at the presynaptic terminal. Upon receiving an action potential, phosphorylation of synapsins, possibly by PKA, leads to the release of synaptic vesicles from cytoskeletal structures. This allows membrane docking and fusion of synaptic vesicles with the presynaptic membrane and neurotransmitter release. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein corresponding to amino acids 362-450 of human Synapsin I
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
RRID
AB_2814568 (BioLegend Cat. No. 853711)
AB_2814569 (BioLegend Cat. No. 853712)

Antigen Details

Structure
Synapsin I is a 704 amino acid protein with an apparent molecular mass of ~74 kD. Synapsin II is a 582 amino acid protein with an apparent molecular mass of ~63 kD. Synapsin III is a 580 amino acid protein with an apparent molecular mass of ~63 kD.
Distribution

Tissue distribution: Mainly in the central nervous system. Also expressed in testis.
Cellular distribution: Cytosol, nucleus, and plasma membrane.

Function
Synapsins are involved in the regulation of synaptic vesicle fusion and neurotransmitter release at the presynaptic terminals.
Interaction
CAPON (Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein), PKA
Cell Type
Neurons
Biology Area
Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Presynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Greengard P, et al. 1993. Science. 259(5096):780
  2. Milovanovic D, et al. 2017. Neuron. 93(5):995
  3. Evergren E, et al. 2007. J. Neurosci. Res. 85(12):2648
Gene ID
6853 View all products for this Gene ID 6854 View all products for this Gene ID 8224 View all products for this Gene ID
UniProt
View information about Synapsin I/II/III on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/14/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account