Alexa Fluor® 488 anti-NF-κB p50 Antibody

Pricing & Availability
Clone
4D1 (See other available formats)
Other Names
NF-κB p50, nuclear factor kappa light chain enhancer in B cells p50, NF-κB1
Isotype
Mouse IgG1, κ
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Product Citations
publications
4D1_NF-kB-p50_082407
Hela cells were stained with Alexa Fluor® 488 anti-NF-kB p50 (4D1) mouse mAb and examined on a fluorescent scope.
  • 4D1_NF-kB-p50_082407
    Hela cells were stained with Alexa Fluor® 488 anti-NF-kB p50 (4D1) mouse mAb and examined on a fluorescent scope.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
616704 100 µg £198
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Description

NF-κB p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. NF-κB is a transcription factor, originally identified as an activator of kappa light chain in B cells. NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation. This 4D1 monoclonal antibody recognizes p105 (precursor of p50) and p50 (active form). 4D1 has been validated for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
recombinant full-length human NF-κB p50
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 4.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence1 on the Amnis ImageStream 100 Imaging Flow Cytometer.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References

(PubMed link indicates BioLegend citation)
  1. Huang F, et al. 2007. J. Immunol. 179:6504. (IF) PubMed
  2. Cao YA, et al. 2008. J. Biol. Chem. 283:15309. PubMed
  3. Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed.
Product Citations
  1. Liu Z, et al. 2011. Mol Cancer Res. 0.727083333. PubMed
  2. Kim S, et al. 2010. Biochem Biophys Res Commun. 391:28. PubMed
  3. Cuadrado E 2018. Immunity. 48:1046. PubMed
Publication Library
RRID
AB_493279 (BioLegend Cat. No. 616704)

Antigen Details

Structure
Member of the Rel/dorsal family. NF-κB p50 is an active form, derived from NF-κB p105, a precursor form.
Distribution

Ubiquitously expressed

Function
A transcription factor, originally identified as an activator of kappa light chain in B cells
Interaction
Interacts with NF-κB p65 and IκB
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Baldwin AS, et al. 1996. Annu. Rev. Immunol. 14:649 (review).
2. Chen F, et al. 1999. Clin. Chem. 45:7 (review).

Regulation
NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation.
Gene ID
4790 View all products for this Gene ID
UniProt
View information about NF-kappaB p50 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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