Alexa Fluor® 488 anti-Myelin Basic Protein Antibody

Pricing & Availability
Clone
SMI 94 (See other available formats)
Other Names
Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_SMI-94_A488_Myelin-Basic-Protein_022619.png
IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No.928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 1_SMI-94_A488_Myelin-Basic-Protein_022619.png
    IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No.928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 2_SMI-94_A488_Myelin_Protein_2_012219.png
    IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein antibody (clone SMI 94) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No.928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
836505 25 µg £74
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836506 100 µg £185
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Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

SMI 94 reacts with the Myelin Basic Protein (MBP) peptic fragment 70-89 of the classic human myelin basic protein sequence.

SMI 94 detects developing and adult myelin, developing oligodendrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons and other cells in brain sections. The combination of SMI 94 with SMI 91 and/or 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

The reactivity of Alexa Fluor® 488 anti-Myelin Basic Protein antibody was validated in FFPE mouse and rat brain tissues.

Application References

(PubMed link indicates BioLegend citation)
  1. Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658. (ICC)
  2. Gensel JC, et al. 2009. J. Neurosci. 29:3956. (IHC-P)
  3. Wang H, et al. 2008. J. Cell. Biol. 182:1171. (ICC, WB)
  4. McLean NA, et al. 2014. PLoS One 9:e110174. (IHC-F)
  5. Tian C, et al. 2014. Front Cell Neurosci. 8:297. (IHC-P)
  6. Wüthrich C, et al. 2009. J. Neuropathol. Exp. Neurol. 68:15. (IHC, ICC)
  7. Maire CL, et al. 2014. Stem Cells 32:313. (IHC-P)
  8. Rusielewicz T, et. al. 2014. Glia 62:580. (ICC)
  9. Li C, et al. 2012. ASN Neuro. 4:e00102. (ICC)
  10. Huang JH, et al. 2009. Tissue Eng. Part A 15:1677. (IHC-P)
  11. Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC)
  12. Zhao X, et al. 2010. Neuron. 65:612. (ICC)

Antigen Details

Structure
Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD.
Distribution

Cytoskeleton, plasma membrane, cytosol, and extracellular.

Function
Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID
4155 View all products for this Gene ID
UniProt
View information about Myelin Basic Protein on UniProt.org

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Go To Top Version: 1    Revision Date: 01/22/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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