- SMI 94 (See other available formats)
- Other Names
- Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
- Mouse IgG1, κ
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- Product Citations
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Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.Product Details
- Human, Mouse, Rat
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-P - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Blue Laser (488 nm)
- Application Notes
SMI 94 reacts with the Myelin Basic Protein (MBP) peptic fragment 70-89 of the classic human myelin basic protein sequence.
SMI 94 detects developing and adult myelin, developing oligodendrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons and other cells in brain sections. The combination of SMI 94 with SMI 91 and/or 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.
The reactivity of Alexa Fluor® 488 anti-Myelin Basic Protein antibody was validated in FFPE mouse and rat brain tissues.
(PubMed link indicates BioLegend citation)
- Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658. (ICC)
- Gensel JC, et al. 2009. J. Neurosci. 29:3956. (IHC-P)
- Wang H, et al. 2008. J. Cell. Biol. 182:1171. (ICC, WB)
- McLean NA, et al. 2014. PLoS One 9:e110174. (IHC-F)
- Tian C, et al. 2014. Front Cell Neurosci. 8:297. (IHC-P)
- Wüthrich C, et al. 2009. J. Neuropathol. Exp. Neurol. 68:15. (IHC, ICC)
- Maire CL, et al. 2014. Stem Cells 32:313. (IHC-P)
- Rusielewicz T, et. al. 2014. Glia 62:580. (ICC)
- Li C, et al. 2012. ASN Neuro. 4:e00102. (ICC)
- Huang JH, et al. 2009. Tissue Eng. Part A 15:1677. (IHC-P)
- Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC)
- Zhao X, et al. 2010. Neuron. 65:612. (ICC)
- Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD.
Cytoskeleton, plasma membrane, cytosol, and extracellular.
- Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Gene ID
- 4155 View all products for this Gene ID
- View information about Myelin Basic Protein on UniProt.org
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