Alexa Fluor® 488 anti-HNRNPA1 Antibody

Pricing & Availability
Clone
4B10/HNRNPA1 (See other available formats)
Other Names
Heterogeneous nuclear ribonucleoprotein A1, hnRNP A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
4B10-HNRNPA1_A488_HNRNPA1_Antibody_1_090919
IHC staining of Alexa Fluor® 488 anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4B10-HNRNPA1_A488_HNRNPA1_Antibody_1_090919
    IHC staining of Alexa Fluor® 488 anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4B10-HNRNPA1_A488_HNRNPA1_Antibody_2_090919
    IHC staining of Alexa Fluor® 488 anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4B10-HNRNPA1_A488_HNRNPA1_Antibody_3_090919
    IHC staining of Alexa Fluor® 488 anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4B10-HNRNPA1_A488_HNRNPA1_Antibody_4_090919
    IHC staining of Alexa Fluor® 488 anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
862507 25 µg £87
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862508 100 µg £185
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Description

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a multi-purpose RNA binding protein involved in RNA metabolism and DNA genomic stability in normal and pathological conditions. hnRNP A1 is ubiquitously expressed and usually associates with nascent RNA polymerase II transcripts. hnRNP A1 is  abundantly expressed in neuronal cells and reproductive tissue. Loss of hnRNP A1 has been observed in several neurodegenerative diseases, including Alzheimer’s disease, multiple sclerosis, amytrophic lateral sclerosis. Structurally, hnRNPA1 has two RNA recognition motifs, which are post-translationally modified to alter its nuclear pore shuttling properties and RNA interaction.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partially purified HeLa HNRNPA1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
RRID
AB_2814597 (BioLegend Cat. No. 862507)
AB_2814598 (BioLegend Cat. No. 862508)

Antigen Details

Structure
hnRNPA1 has two isoforms. Human HNRPNA1 isoform A1-A is a 320 amino acid protein with a molecular mass of 34 kD, and isoform A1-B is a 372 amino acid protein with a molecular mass of 38 kD.
Distribution

Tissue Distribution: Ubiquitous expression
Cellular Source: Nucleus and cytoplasm

Function
RNA metabolism, genomic stability
Interaction
Nascent RNA polymerase II transcripts
Cell Type
Neurons
Biology Area
Neurodegeneration, Neuroscience, Protein Synthesis
Antigen References
  1. Bekenstein U, et al. 2013. Mol Cell Neurosci. 56:436-46.
  2. Jean-Philippe J, et al. 2014. Biochim Biophys Acta. 1839(4): 251–258.
Gene ID
3178 View all products for this Gene ID 15382 View all products for this Gene ID 29578 View all products for this Gene ID
UniProt
View information about HNRNPA1 on UniProt.org

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Go To Top Version: 1    Revision Date: 09/09/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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