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Alexa Fluor® 488 anti-Giantin Antibody (Previously Covance catalog# A488-114L)

Pricing & Availability
Clone
Poly19087 (See other available formats)
Regulatory Status
RUO
Other Names
Golgin subfamily B member 1, macrogolgin, golgi integral membrane protein 1, 372 kD Golgi complex-associated protein, golgin B1, golgi integral membrane protein, golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1
Previously
Covance Catalog# A488-114L
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
AF488_Giantin_Antibody_IHC_022815
Formalin fixed HeLa cells stained with Alexa Fluor™ 488 Giantin used at a dilution of 1:1000.
  • AF488_Giantin_Antibody_IHC_022815
    Formalin fixed HeLa cells stained with Alexa Fluor™ 488 Giantin used at a dilution of 1:1000.
  • Poly19087_A488_giantin_Antibody_2_012218
    Immunofluorescence of A431 cells with (A) Rabbit Isotype control (Negative, Cat. No. 910801) or (B, C) Alexa Fluor® 488 Giantin antibody (Poly19087). Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/10, and (B, C) is 1/30. Concentrations for (A, B) is 4 µg/ml, (C) is 2 µg/ml.
See Alexa Fluor® 488 spectral data
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908701 100 µL £238
 
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Description

Giantin is a membrane-inserted component of the cis and medial Golgi with a large rod-like cytoplasmic domain. This antibody serves as an excellent marker for the Golgi complex in mammalian cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Non-Human Primates, Mouse, Rat, Dog
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This polyclonal antibody was raised against the N-terminus, residues 1-469, of human giantin.
Formulation
Phosphate-buffered solution + 50% glycerol + 2mM Sodium Azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
1 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution range of 1:250 - 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This antibody is effective in immunoblotting (WB) and immunocytochemistry (ICC).

*Predicted MW = 367 kD

Western transfer should be conducted in the presence of 0.1% SDS. After transfer, rinse blot with 50% MeOH to remove any residual SDS.

This antibody is covalently coupled to Alexa Fluor® 488 to generate a one-step staining reagent; no secondary antibody detection is required. Alexa Fluor® 488 is an excellent alternative to fluorescein (FITC) dyes.

Application References

(PubMed link indicates BioLegend citation)
  1. Linstedt AD, Hauri HP. 1993. Mol Biol Cell. 4:679.
  2. Kong S, et al. 2013. Cell Res. 23:835. (IF) PubMed
Product Citations
  1. Cha JH et al. 2018. Molecular cell. 71(4):606-620 . PubMed
  2. Lam MT, et al. 2019. J Exp Med. 216:2778. PubMed
  3. Kong S, et al. 2013. Cell Res. 23:835-850. PubMed
  4. Cabanes-Creus M, et al. 2021. Mol Ther Methods Clin Dev. 21:607. PubMed
RRID
AB_2565089 (BioLegend Cat. No. 908701)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Golgi Markers
Gene ID
2804 View all products for this Gene ID
UniProt
View information about Giantin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 09/22/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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