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Alexa Fluor® 488 anti-GAPDH Antibody

Pricing & Availability
Clone
W17079A (See other available formats)
Regulatory Status
RUO
Other Names
glyceraldehyde-3-phosphate dehydrogenase
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17079A_A488_GAPDH_Antibody_1_ICC_062618
HeLa cells were fixed with cold methanol for 10 minute and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with (A) 1: 250 diluted (2 µg/ml) Alexa Fluor® 594 rat IgG2a, κ Isotype Ctrl antibody or with Alexa Fluor® 488 anti-GAPDH antibody (green) at (B) 1: 250 (2 µg/ml), (C) 1:500 (1 µg/ml) and (D) 1: 1000 (0.5 µg/ml) dilutions. Nuclei were counterstained with DAPI (blue). The images were captured with a 60X objective.
  • W17079A_A488_GAPDH_Antibody_1_ICC_062618
    HeLa cells were fixed with cold methanol for 10 minute and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4°C with (A) 1: 250 diluted (2 µg/ml) Alexa Fluor® 594 rat IgG2a, κ Isotype Ctrl antibody or with Alexa Fluor® 488 anti-GAPDH antibody (green) at (B) 1: 250 (2 µg/ml), (C) 1:500 (1 µg/ml) and (D) 1: 1000 (0.5 µg/ml) dilutions. Nuclei were counterstained with DAPI (blue). The images were captured with a 60X objective.
Compare all formats See Alexa Fluor® 488 spectral data
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607905 25 µg £94
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607906 100 µg £230
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Description

GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein (161-335 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This clone weakly reacts with rat GAPDH.

RRID
AB_2734506 (BioLegend Cat. No. 607905)
AB_2734507 (BioLegend Cat. No. 607906)

Antigen Details

Structure
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution

Ubiquitously expressed, locate in cytoplasm and perinuclear region.

Function
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Interaction
Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2.
Biology Area
Cell Biology
Antigen References
  1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
  2. Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460.
  3. Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527.
  4. Zheng L, et al. 2003. Cell. 114:255.
  5. Wang Q, et al. 2005. FASEB J. 19:869.
  6. Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405.
Gene ID
5590 View all products for this Gene ID
UniProt
View information about GAPDH on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/26/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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