Loading...

Alexa Fluor® 488 anti-DYKDDDDK Tag Antibody

Pricing & Availability
Clone
L5 (See other available formats)
Regulatory Status
RUO
Other Names
FLAG tag
Isotype
Rat IgG2a, λ
Ave. Rating
Submit a Review
Product Citations
publications
L5_A488_DYKDDDDK_ICC_Antibody_032918
HeLa cells non-transfected (A) or transfected with DYKDDDDK Tag fused protein (B and C) were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees with 1: 250 diluted (2 µg/ml, Figure A and B) and 1: 1000 diluted (0.5 µg/ml) Alexa Fluor® 488 anti- DYKDDDDK Tag (Figure C, green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • L5_A488_DYKDDDDK_ICC_Antibody_032918
    HeLa cells non-transfected (A) or transfected with DYKDDDDK Tag fused protein (B and C) were fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained overnight at 4 degrees with 1: 250 diluted (2 µg/ml, Figure A and B) and 1: 1000 diluted (0.5 µg/ml) Alexa Fluor® 488 anti- DYKDDDDK Tag (Figure C, green). Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
637317 25 µg £109
Check Availability


Need larger quantities of this item?
Request Bulk Quote
637318 100 µg £254
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
DYKDDDDK-tagged mouse Langerin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.

Application References

(PubMed link indicates BioLegend citation)
  1. Park SH, et al. 2008. J Immunol Methods. 331:27.
  2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
  3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
  4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
  5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
  6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
Product Citations
  1. Wang J, et al. 2022. Front Immunol. 13:863346. PubMed
  2. Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed
  3. Martin-Sancho L, et al. 2021. Mol Cell. 81:2656. PubMed
  4. Martin-Sancho L, et al. 2020. bioRxiv. . PubMed
  5. Gallo E, et al. 2020. MAbs. 12:1717265. PubMed
RRID
AB_2728470 (BioLegend Cat. No. 637317)
AB_2810690 (BioLegend Cat. No. 637318)

Antigen Details

Biology Area
Cell Biology
Antigen References

1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754.

Gene ID
NA
UniProt
View information about DYKDDDDK on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/21/2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-DYKDDDDK Tag

  • Anti-DYKDDDDK Tag (L5) Affinity Gel

  • APC anti-DYKDDDDK Tag

  • PE anti-DYKDDDDK Tag

  • Direct-Blot™ HRP anti-DYKDDDDK Tag

  • Alexa Fluor® 594 anti-DYKDDDDK Tag

  • Alexa Fluor® 647 anti-DYKDDDDK Tag

  • Alexa Fluor® 488 anti-DYKDDDDK Tag

  • PE/Cyanine7 anti-DYKDDDDK Tag

  • Brilliant Violet 421™ anti-DYKDDDDK Tag

  • PerCP/Cyanine5.5 anti-DYKDDDDK Tag

  • Ultra-LEAF™ Purified anti-DYKDDDDK Tag

  • PE/Dazzle™ 594 anti-DYKDDDDK Tag Antibody

  • TotalSeq™-B1129 anti-DYKDDDDK Tag

  • TotalSeq™-A1129 anti-DYKDDDDK Tag

  • TotalSeq™-C1129 anti-DYKDDDDK Tag

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account