Alexa Fluor® 488 anti-Cytokeratin (pan reactive) Antibody

Pricing & Availability
C-11 (See other available formats)
Regulatory Status
Other Names
Cytokeratin, Keratin
Mouse IgG1, κ
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Product Citations
MCF-7 mammary adenocarcinoma cells were fixed, permeabilized and blocked according to standard protocols. The cells were stained with anti-Cytokeratin (pan reactive) (clone C-11) Alexa Fluor® 488 (green) antibody. Nuclei were counterstained with DAPI (blue).
  • Cdash11_A488_Cytokeratin_PanReactive_Antibody_1_ICC_080619
    MCF-7 mammary adenocarcinoma cells were fixed, permeabilized and blocked according to standard protocols. The cells were stained with anti-Cytokeratin (pan reactive) (clone C-11) Alexa Fluor® 488 (green) antibody. Nuclei were counterstained with DAPI (blue).
  • Cdash11_A488_Cytokeratin_PanReactive_Antibody_2_IHC-P_080619
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffination, rehydration, antigen retrieval, and blocking. Tissue was stained with anti-Pan Cytokeratin (clone C-11) Alexa Fluor® 488 (green) antibody and anti-mouse/human CD44 (clone IM7) Alexa Fluor® 594 (red) antibody. Nuclei were counterstained with DAPI (blue). Expanded protocol available by following the Additional Data link.
Compare all formats See Alexa Fluor® 488 spectral data
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628608 100 µg £238
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Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Keratin enriched fraction from human epidermoid carcinoma.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), immunocytochemistry, and spatial biology (IBEX)7,8.

Application References

(PubMed link indicates BioLegend citation)
  1. Kovarik J. 1988. Int. J. Cancer Suppl. 3:50.
  2. Bartek J, et al. 1991. J. Pathol. 164:215.
  3. Chernyavsky A I, 2007. J. Biol. Chem. doi:10.1074/jbc.M611365200. PubMed
  4. Comito G, et al. 2012. Cancer Lett. 324:31. PubMed
  5. Vojt-òsek B, et al. 1989. Folia Biol (Praha). 35:373. (reactivity with rat, dog, sheep, pig, cow)
  6. Dekaney CM, et al. 2005. Gastroenterology 129:1567. (ICC)
  7. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  8. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bordignon P et al. 2019. Cell Rep. 28(9):2358-2372 . PubMed
  2. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  3. Yee–de León JF, et al. 2020. Sci Rep. 5.654861111. PubMed
  4. Alzubi MA, et al. 2019. Breast Cancer Res. 21:36. PubMed
  5. Soto-Pantoja DR, et al. 2021. Cancer Res. 81:3890. PubMed
  6. Potter DS, et al. 2021. Cell Death Dis. 12:741. PubMed
  7. Aguilar–Avelar C, et al. 2019. Sci Rep. 9:13766. PubMed
AB_2616664 (BioLegend Cat. No. 628608)

Antigen Details

Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits

Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus

Intermediate filament protein involved with the cytoskeleton.
Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3875 View all products for this Gene ID
View information about Cytokeratin pan reactive on

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Go To Top Version: 5    Revision Date: 12/17/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Cytokeratin (pan reactive)

  • Alexa Fluor® 647 anti-Cytokeratin (pan reactive)

  • Alexa Fluor® 594 anti-Cytokeratin (pan reactive)

  • Alexa Fluor® 488 anti-Cytokeratin (pan reactive)


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