Alexa Flour® 594 anti-p53 Antibody

Pricing & Availability
Clone
DO-1 (See other available formats)
Other Names
Tumor protein p53 (TP53)
Isotype
Mouse IgG2a, κ
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Product Citations
publications
DO-1_A594_p53_Antibody_1_070519
A431 cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:250 dilution (2 µg/mL) of either Alexa Fluor® 594 Mouse IgG2a, κ Isotype Control Antibody (Cat. No. 400280, panel A) or Alexa Fluor® 594 anti-p53 Antibody (panel B) for 2 hours at room temperature. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
  • DO-1_A594_p53_Antibody_1_070519
    A431 cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:250 dilution (2 µg/mL) of either Alexa Fluor® 594 Mouse IgG2a, κ Isotype Control Antibody (Cat. No. 400280, panel A) or Alexa Fluor® 594 anti-p53 Antibody (panel B) for 2 hours at room temperature. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
645705 25 µg £81
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645706 100 µg £185
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Description

p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
P53 N-terminal linear epitope aa 20-25
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application References

(PubMed link indicates BioLegend citation)
  1. Vojtesek B, et al. 1992. J Immuno Methods 151:237
  2. Stephen CW, et al. 1995. J Mol Biol 248:58

Antigen Details

Structure
Tetramer; 53 kD
Distribution

Nuclear when activated

Function
Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity
Interaction
mdm2, mdmx, TopoI, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, DNA Repair/Replication, Stem Cells, Transcription Factors
Antigen References

1. Vogelstein B, et al. 1992. Cell. 70:523.
2. Shieh S, et al. 1997. Cell. 91:325.
3. Mihara M, et al. 2003. Mol. Cell 11:577.
4. Saito S, et al. 2003. J. Biol. Chem. 278:37536.

Gene ID
7157 View all products for this Gene ID
UniProt
View information about p53 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/05/2019

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