Product: Purified anti-mouse IFNAR-1

Catalog No.: 127302

Adaeze Izuogu, Graduate Research Assistant The University of Toledo HSC

Purified anti-mouse IFNAR-1
Overall:	Product Quality:	Ease of Use:
Not much success in the species tested.

Experimental Design

Application:	Western Blotting
Cells used:	C57BL/6 and Peromyscus cells
Brief Protocol:	I ran cell lysates on a gel and blotted for the presence of IFNAR1 in my cells of interest. I tested the antibody at various dilutions: 1:1000, 1:500 and 1:200.
Results Summary:	This antibody did not detect IFNAR1 in the cells I tested, which I find surprising because I expected to detect protein in the control (C57BL/6) cells.
Additional Notes:	I knew that I was taking a chance with the peromyscus cells because the protein may be so different between both species that the antibodies wouldn't work. However, I expected to detect IFNAR1 in the C57BL/6 cells.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

Technical Service Notes

Clone MAR1-5A3 has not been tested in-house for western blot applications. Rather, it's use in WB has been noted by others who published on it (i.e., Dunn GP, et al. 2005. Nat. Immunol. 6:722). If you feel the product has not been useful for your studies, please feel free to contact tech@biolegend.com regarding a possible credit for your account.

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