Product: Pacific Blue™ anti-human CD326 (EpCAM) |
Catalog No.: 324218 |
Brian Bierie, Postdoc WIBR |
EpCAM is Detected on Basal Mammary Carcinoma Cells with Epithelial Traits | |||
Overall: | Product Quality: | Ease of Use: | |
EpCAM was detected on 99% of HCC1806 cells. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | HCC1806 |
Brief Protocol: | Cells were treated with trypsin and quenched using DMEM + 10%FBS. Pellets were re-suspended at 1x106 cells per 100ul of ice cold PBS + 2% IFS. Antibody was used at 1:100 and incubated on ice for 30 minutes. Cells were then washed with a 10:1 volume of ice cold PBS + 2% IFS. Pellets were re-suspended in ice cold PBS + 2% IFS to a volume of 500ul. |
Results Summary: | EpCAM was detected on 99% of HCC1806 cells. |
Additional Notes: |
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