Product: Treg-Protector™ (anti-ARTC2 Nanobody)

Catalog No.: 149801

Rissiek Björn, Dr. University Medical Center Hamburg-Eppendorf

Treg-Protector Protects your Tregs During Cell Preparation
Overall:	Product Quality:	Ease of Use:
Preserves vitality of mouse Tregs and also NKT cells after cell preparation.

Experimental Design

Application:	Improved preparation of mouse regulatory T cells
Cells used:	Primary mouse spleen Tregs
Brief Protocol:	The s+16a clone specifically blocks the ecto-enzyme ARTC2 on mouse T cells when injected 15min prior to sacrificing the mouse (50µg i.v.). This prevents ADP-ribosylation of T cell surface proteins including the P2X7 ion channel. ADP-ribosylation of P2X7 occurs at 4°C, however, when isolated T cells are brought back to 37 °C this post-translational modification induces opening of the ion channel subsequently inducing cell death.
Results Summary:	Injection of Treg-Protector preserves the vitality of freshly prepared Tregs retaining their suppressive capacity e.g. during in vitro suppression assays.
Additional Notes:

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Publication Status This data has been published. Pubmed

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