Product: True-Nuclear™ Transcription Factor Buffer Set

Catalog No.: 424401

Jacqueline Kimmey, Graduate Student Washington University

OK (True-Nuclear™ Transcription Factor Buffer Set)
Overall:	Product Quality:	Ease of Use:
Foxp3 perm buffer worked better for my cells, this perm buffer made everything look weirdly positive.

Experimental Design

Application:	Flow Cytometry - Ki-67 staining
Cells used:	Bone Marrow cells (murine)
Brief Protocol:	Cells were prepped from bone marrow and grown in vitro (making BMDM). Cells were stained with surface Ab and then fixed and permed as per product guidelines. Cells were then stained with Ki-67 (nuclear) and analyzed by flow cytometry.
Results Summary:	Didn't work - Foxp3 perm buffer worked better for me.
Additional Notes:	The populations I have gated are weird, because I took bone marrow cells that were grown in the presence of M-CSF to make BMDM. I used these cells because they were convenient at the time, just to test the perm results. So the fact that I have M-CSF positive neutrophils is an artifact of the system. This buffer gave a positive population (that looks like I had my laser set too high, but if I lowered that, the whole population became negative, there wasn't the nice distinction between a positive and negative population as seen with Foxp3 buffer. In the attached image, the top two rows were stained with Foxp3 perm buffer, the bottom two were stained with True-Nuclear™ Buffer. May be good for other nuclear stains, but for Ki-67, it didn't work in my hands.

Data Image Click on the image for full size data.

Publication Status This data has not been published.

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