Product: Alexa Fluor® 594 Goat anti-rat IgG (minimal x-reactivity)

Catalog No.: 405422

yougang zhai, research associate Loyola University Chicago

Indirect Staining of Mouse Intestinal Epithelial Cells (Alexa Fluor® 594 Goat anti-rat IgG (minimal x-reactivity))
Overall:	Product Quality:	Ease of Use:
This antibody generated very strong signal for IFA analysis.

Experimental Design

Application:	Immunofluorescence
Cells used:	Mouse intestine tissue
Brief Protocol:	Intestinal tissue were collected from mice and snap frozen and cryo sections were used for staining the intestinal epithelial cells. Sections were fixed and then blocked in 5% FBS buffer. Then, sections were stained with Anti-EpCAM antibody followed by Alexa Fluor® 594 anti-rat IgG staining. Slides were coverslipped with DAPI containing mounting medium and analyzed under fluorescence microscopy.
Results Summary:	Intestinal epithelial cells were stained with very strong and specific signal.
Additional Notes:	This antibody generated very strong and specific signals are very good for IFA experiment.

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Publication Status This data has not been published.

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