Product: PE anti-human CD319 (CRACC) |
Catalog No.: 331806 |
Jong Kim, Post-doc Emory University |
Anti-human CD319-PE Antibody | |||
Overall: | Product Quality: | Ease of Use: | |
It stains activated monocytes. |
Experimental Design |
Application: | Flow cytomery |
Cells used: | Human monocytes activated by TLR agonists |
Brief Protocol: | 1. Human monocytes were purified by FACS (CD14+) or magnetic depletion. 2. Monocytes were Fc receptor blocked. 3. Stained with anti-CD319-PE or isotype control-PE. 4. Run on flow cytometer. |
Results Summary: | Activated monocytes express CD319 within 24 hours. |
Additional Notes: | CD319 antibody also acts as agonist antibody. Crosslinking with secondary antibody triggers signaling through CD319. |
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