Application: |
Immunofluorescence |
Cells used: |
MDA-MD-231 human breast cancer cells |
Brief Protocol: |
Cells transfected with non-targeting or beta1 integrin targeting si-RNA. 48hr after transfection, fixation with 4% paraformaldehyde for 15min. Permeabilisation with 0.25% Triton-X100 Blocking with 5% BSA Staining with 2ug/ml antibody in PBS/5% BSA for 1hr at RT |
Results Summary: |
The signal is very strong in the non-targeting siRNA condition and is significantly reduced upon beta1 integrin knock-down. |
Additional Notes: |
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