Product: APC/Cyanine7 anti-mouse CD326 (Ep-CAM) |
Catalog No.: 118217 |
Marie-Eve Lebel, postdoctoral fellow Maisonneuve Rosemont Hospital Research center |
APC/Cyanine7 anti-mouse CD326 (Ep-CAM) - Ep-CAM Staining of Thymic Epithelial Cells | |||
Overall: | Product Quality: | Ease of Use: | |
Good discrimination of thymic epithelial cells. |
Experimental Design |
Application: | Flow cytometry |
Cells used: | Thymic epithelial cells |
Brief Protocol: | Cells suspension of thymic cells from C57BL/6 was obtained after digestion with collagenase D, DNase I and Papain for 15 minutes at 37 degrees. 5 x 10^6 cells were stained with 100ul of 1:200 dilution Ep-CAM APC-Cyanine7 and 1:200 anti-CD45.2 A700 (Cat#109822) for 20 minutes at 4 degrees. |
Results Summary: | Good discrimination of CD45- Ep-CAM+ thymic epithelial cells. |
Additional Notes: |
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