Product: ELISA MAX™ Deluxe Set Mouse IL-12 (p70)
Catalog No.: 433604
Julia Dickow, M.Sc. Essen University Hospital

Mouse IL-12 (p70) ELISA MAX™ Deluxe
Overall: Product Quality: Ease of Use:
Good working ELISA for quantification of cytokine production.
Experimental Design

Application: ELISA
Cells used: In vitro cultured CD8 T cells with DCs
Brief Protocol: Dilute capture antibody 1:200 in 1 x coating buffer and incubate over night at 4 °C.
Next day, wash plate 4 times with 200 µl of 1 x Wash Buffer (PBS + 0,05 % Tween-20).
Block plate by adding 200 µl of Assay Diluent for 1hour.
Wash plate 4 times.
Add 100 µl diluted standards, as well as (serial-diluted) samples and incubate for 2 hours.
Wash plate 4 times.
Add 200 µl of diluted detection antibody (1:200 in Assay Diluent) and incubate for 1 hour.
Wash plate 4 times.
Add 200 µl of diluted Avidin-Antibody (1:1000 in Assay Diluent) and incubate for 30 minutes.
Wash plate 5 times.
Add 100 µl of TMB Substrate, incubate for30 minutes and then stop the reaction with 2 N H2SO4.
Measure absorbance at 570 nm (reference filter 450 nm).
Results Summary: Suitable for measurement of produced IL-12 by cells cultured in vitro.
Additional Notes:
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